Comparative genomic analysis of male and female poplars

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Elena Pushkova¹, George Krasnov², Roman Novakovskiy³, Nadezhda Bolsheva⁴, Nataliya Melnikova⁵, Alexey Dmitriev^6
1Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, pushkova18@gmail.com
²Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, gskrasnov@mail.ru
³Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, 0legovich46@mail.ru
⁴Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, nlbolsheva@mail.ru
⁵Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, mnv-4529264@yandex.ru
⁶Engelhardt Institute of Molecular Biology, RAS, Moscow, Russia, alex_245@mail.ru

In the genomic analysis of trees, species belonging to the Populus genera serve as model objects. In our work, we carried out sequencing of the genome of male and female plants of Populus x sibirica, which are widespread in the Moscow region. Achieve high quality de novo In assemblies, we used a combination of two platforms, namely Oxford Nanopore and Illumina, to obtain long and high-precision short read operations, respectively. In general, using the MinION sequencer, we got 18 GB with an average N50 of 25 kb for each genotype, while using the HiSeq 2500 tool, we got from 36 to 52 million paired 125 + 125 readings for male and female plants. The Shasta assembler provided the best assembly results: N50 values ​​were around 0.5 MB, and integrity was above 95% according to BUSCO. Analysis of the high-quality genomes of male and female P. x sibirica trees makes it possible to identify genes associated with poplar sex, which will facilitate the determination of its sex determination mechanism.