Genes expression related to the effects of hypoxia in the marine mussel, Mytilus galloprovincialis

Ekaterina Vodiasova¹, Aleksandra Andreyeva², Рђnastasiya Lantushenko³, Yakov Meger⁴, Irina Deghtyar⁵, Dmitry Afonnikov^6
1IBSS RAS, Sevastopol, Russia, eavodiasova@gmail.com
²IBSS RAS, Sevastopol, Russia, andreevaal@gmail.com
³SSU, Sevastopol, Russia, lantushenko@mail.ru
⁴SSU, Sevastopol, Russia, meger_yakov@mail.ru
⁵SSU, Sevastopol, Russia, skuratovskaya95@mail.ru
⁶ICG SB RAS, Novosibirsk, Russia, ada@bionet.nsc.ru

Despite that mussel is an important object of mariculture and can play the role of an ecosystem bio-indicator, the study of the effect of hypoxia on these mollusСЃs is limited only by biochemical and RT-PCR experiments for individual genes (enzymes). There is no data on changes in the expression profile of all genes based on RNA sequencing. At the same time, an understanding of physiological processes requires comprehensive studies based on the study of the expression of all genes in target tissues. At this work, the transcriptomes of the gills of Mytilus galloprovincialis exposed to hypoxic stress (DO 0.3 mg/l) for 24h were sequenced using Illumina technology for the first time. The raw reads assembled into 255,302 transcripts with an N50 value of 1,273 nt. A total of 65 transcripts that differed in abundance in the hypoxia-exposed mussels were identified. Their putative function was assigned using BLAST. The data obtained will allow further large-scale studies for other targeted tissues and to study all aspects of the physiological response of M. galloprovincialis to the emerging oxygen deficiency in the environment.