Interplay between 5-HT and BDNF system in recombinant mouse strain upon chronic fluoxetine administration

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Rodnyy A.Ya.¹, Kondaurova E.M², Antonov Y.V.³, Ilchibaeva T.V.⁴, Tsybko A.S.⁵, Naumenko V.S.^6
1Institute of Cytology and Genetics Novosibirsk, Russia, aleksandr1994rodny@gmail.com
²Institute of Cytology and Genetics Novosibirsk, Russia, kond_em@bionet.nsc.ru
³Institute of Cytology and Genetics Novosibirsk, Russia, yegor@bionet.nsc.ru
⁴Institute of Cytology and Genetics Novosibirsk, Russia, rbicehok@mail.ru
⁵Institute of Cytology and Genetics Novosibirsk, Russia, antontsybko@bionet.nsc.ru
⁶Institute of Cytology and Genetics Novosibirsk, Russia, naumenko2002@bionet.nsc.ru

BDNF plays a key role in the development, differentiation, synaptogenesis and survival of brain neurons and in the processes of their adaptation to external impacts. Serotonergic (5-HT) system is another basic player in brain development and neuroplasticity. The study presents a comparative analysis of chronic fluoxetine treatment in recombinant mice differing in distal chromosome 13 fragment containing Htr1A gene of CBA mice strain on C57Bl6 genetic background. The problem here to be studied is mechanism of BDNF and 5-HT systems` interactions in antidepressant insensitivity. We measured mRNA and protein levels of BDNF, p75^NTR, TrkB, 5-HT1A and 5-HT7 receptors, levels of 5-HT and its primary metabolite 5-Hydroxyindoleacetic acid (5-HIAA) in the brain structures that could have play primary role in mechanism of depression – frontal cortex and hippocampus. At the heart of the discussion are the different changes in BDNF system as well as in 5-HT system which allows us to conclude that the chronic fluoxetine injection increased depressive-like behavior in recombinant mice carrying distal chromosome 13 fragment containing Htr1A gene of CBA mice.