Poster (download)
255Alexandr Vikhorev1, Nikolay Shmakov2, Anastasia Glagoleva3, Elena Khlestkina4, Olesya Shoeva5
1Novosibirsk State University, vikhorev@bionet.nsc.ru
2Institute of Cytology and Genetics SB RAS, shmakov@bionet.nsc.ru
3Institute of Cytology and Genetics SB RAS, glagoleva@bionet.nsc.ru
4All-Russian Institute of Plant Recources, khlest@bionet.nsc.ru
5Institute of Cytology and Genetics SB RAS, olesya_ter@bionet.nsc.ru
Bread wheat (Triticum aestivum L.) is the most important crop in the world. It provides about 20% of the total calories consumed by humans. For a long time, wheat selection was mainly based on phenotypic traits of the shoot, but the roots were given little attention. As a result, the root system of modern wheat varieties has weakened. Therefore, the study of genetic control of wheat roots development is an urgent issue. In this study, sequencing of RNA libraries from roots and coleoptile of Russian spring variety “Saratovskaya, 29” was performed. De novo transcriptome was assembled. 31,488 up-regulated, 35,851 down-regulated and 18,040 roots-specific transcripts were found. The subsequent analysis of genes with differential expression will allow choosing the candidate genes for development of wheat varieties with resistant root system.
Pretty pictures, dear Alexander, thank you for the interesting topic. I have a few questions. As you know, edgeR proposes two tests for identification DEGs – quasi-likelihood ratio test and likelihood ratio test. What test have you used and why? I’m a little confused by the results of the functional annotation because I don’t see the processes associated with the root development? In this case, how will you look for the genes associated with root development and potentially related to the root resistance?