{"id":10071,"date":"2020-06-29T09:36:52","date_gmt":"2020-06-29T09:36:52","guid":{"rendered":"https:\/\/bgrssb.icgbio.ru\/2020\/2020\/06\/29\/hydroxymethylation-changes-during-early-embryonic-development-in-zebrafish\/"},"modified":"2020-06-29T09:36:52","modified_gmt":"2020-06-29T09:36:52","slug":"hydroxymethylation-changes-during-early-embryonic-development-in-zebrafish","status":"publish","type":"post","link":"https:\/\/bgrssb.icgbio.ru\/2020\/2020\/06\/29\/hydroxymethylation-changes-during-early-embryonic-development-in-zebrafish\/","title":{"rendered":"Hydroxymethylation changes during early embryonic development in zebrafish"},"content":{"rendered":"<p>Artem Nedoluzhko<sup>1<\/sup>, Paula Berrutti<sup>2<\/sup>, Igo Guimar\u0413\u0408es<sup>3<\/sup>, Ioannis Konstantinidis<sup>4<\/sup>, Igor Babiak<sup>5<\/sup>, Jorge M.O. Fernandes<sup>6<\/sup><br \/><sup>1<\/sup>Nord University, artem.nedoluzhko@nord.no<br \/><sup>2<\/sup>Nord University, paula.d.berrutti@nord.no<br \/><sup>3<\/sup>Universidade Federal de Goi\u0413\u040es, igoguimaraes@ufg.br<br \/><sup>4<\/sup>Nord University, ioannis.konstantinidis@nord.no<br \/><sup>5<\/sup>Nord University, igor.s.babiak@nord.no<br \/><sup>6<\/sup>Nord University, jorge.m.fernandes@nord.no<\/p>\n<p class=\"Abstract\"><span lang=\"EN-US\">A large number of studies in organisms across the eukaryotic phyla have shown that DNA 5-methylcytosine (5mC) modification is one of many mechanisms that suppress gene expression. <\/span><\/p>\n<p class=\"Abstract\"><span lang=\"EN-US\">Cytosine hydroxymethylation has been described during the past years; this DNA modification is increasingly recognized as an important component of epigenetic regulation in eukaryotes. <\/span><\/p>\n<p class=\"Abstract\"><span lang=\"EN-US\">In the present study, we investigated if hydroxymethylation may be involved in fish embryonic development and demonstrated for the first time at a genome-wide level and single nucleotide resolution the hydroxymethylome changes during zebrafish (<i>Danio rerio<\/i>) embryogenesis from one-cell stage to hatching. DNA hydroxymethylation was profiled by reduced representation hydroxymethylation profiling (RRHP), as shortly described in Fig. 1.<\/span><\/p>\n<p class=\"Abstract\"><span lang=\"EN-US\">Taken together with recently published data on 5-methylcytosine (5mC) modification events in <i>D. rerio<\/i>, our data unveil a new role for DNA hydroxymethylation in epigenetic regulation of fish embryonic development.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Artem Nedoluzhko1, Paula Berrutti2, Igo Guimar\u0413\u0408es3, Ioannis Konstantinidis4, Igor Babiak5, Jorge M.O. Fernandes61Nord University, artem.nedoluzhko@nord.no2Nord University, paula.d.berrutti@nord.no3Universidade Federal de Goi\u0413\u040es, igoguimaraes@ufg.br4Nord University, ioannis.konstantinidis@nord.no5Nord University, igor.s.babiak@nord.no6Nord University, jorge.m.fernandes@nord.no A large number of studies in organisms across the eukaryotic phyla have shown that DNA 5-methylcytosine (5mC) modification is one of many mechanisms that suppress gene expression. Cytosine hydroxymethylation has been described during the past years; this DNA modification is increasingly recognized as an important component of epigenetic regulation in eukaryotes. In the present study, we investigated if hydroxymethylation may be involved in fish embryonic development and demonstrated for the first time at a genome-wide level and single nucleotide resolution the hydroxymethylome changes during zebrafish (Danio rerio) embryogenesis from one-cell stage to hatching. DNA hydroxymethylation was profiled by reduced representation hydroxymethylation profiling (RRHP), as shortly described in Fig. 1. Taken together with recently published data on 5-methylcytosine (5mC) modification events in D. rerio, our data unveil a new role for DNA hydroxymethylation in epigenetic regulation of fish embryonic development.<\/p>\n","protected":false},"author":9,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[2171],"tags":[1428,1430,1429,1431,1427],"_links":{"self":[{"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/posts\/10071"}],"collection":[{"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/comments?post=10071"}],"version-history":[{"count":0,"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/posts\/10071\/revisions"}],"wp:attachment":[{"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/media?parent=10071"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/categories?post=10071"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/tags?post=10071"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}