{"id":10148,"date":"2020-06-29T09:36:52","date_gmt":"2020-06-29T09:36:52","guid":{"rendered":"https:\/\/bgrssb.icgbio.ru\/2020\/2020\/06\/29\/proteomic-analysis-of-extracellular-vesicles-produced-by-placental-mesenchymal-stem-cells\/"},"modified":"2020-06-29T09:36:52","modified_gmt":"2020-06-29T09:36:52","slug":"proteomic-analysis-of-extracellular-vesicles-produced-by-placental-mesenchymal-stem-cells","status":"publish","type":"post","link":"https:\/\/bgrssb.icgbio.ru\/2020\/2020\/06\/29\/proteomic-analysis-of-extracellular-vesicles-produced-by-placental-mesenchymal-stem-cells\/","title":{"rendered":"Proteomic Analysis of Extracellular Vesicles Produced by Placental Mesenchymal Stem Cells"},"content":{"rendered":"

Anastasia Khutornenko1<\/sup>, Anastasia Zharikova2<\/sup>, Vasily Popkov3<\/sup>, Sergey Kovalchuk4<\/sup>, Kirill Goryunov5<\/sup>, Yulia Shevtsova6<\/sup>, Egor Plotnikov7<\/sup>, Dmitry Zorov8<\/sup>, Denis Silachev9<\/sup>
1<\/sup>Laboratory of Cell Technologies, Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia, bioingenier@gmail.com
2<\/sup>Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russia, azharikova89@gmail.com
3<\/sup>The A.N. Belozersky Institute Of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia, popkov.vas@gmail.com
4<\/sup>Laboratory of Bioinformatic methods for Combinatorial Chemistry and Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia, xerx222@gmail.com
5<\/sup>Laboratory of Cell Technologies, Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia, kirishgor@gmail.com
6<\/sup>Laboratory of Cell Technologies, Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia, yulshevtsova@yandex.ru
7<\/sup>A.N. Belozersky Institute of Physico-Chemical Biology; Research Center of Obstetrics, Gynecology and Perinatology, Moscow, Russia, plotnikov@belozersky.msu.ru
8<\/sup>A.N. Belozersky Institute of Physico-Chemical Biology; Reserch Center of Obstetrics, Gynecology and Perinatology, Moscow, Russia, zorov@belozersky.msu.ru
9<\/sup>A.N. Belozersky Institute of Physico-Chemical Biology; Research Center of Obstetrics, Gynecology and Perinatology, Moscow, Russia, d_silachev@oparina4.ru<\/p>\n

Mesenchymal stem\/stromal cells (MSCs) have therapeutic potential in many pathological conditions, that is explained mostly by their paracrine action. MSCs secrete extracellular vesicles (EVs) packed with proteins, DNA, RNA and lipids. One of the easily accessible and reach sources of MSCs is placenta. In order to reveal possible pathways, by which EVs produced by placenta-derived MSCs (EVs-PMSC) could realize their therapeutic potential we conducted proteomic analysis of EVs-PMSC and subsequent bioinformatic analysis of identified proteins using Reactome pathway database. Identified peptides were mapped to 2093 proteins. Among the 2093 proteins, 972 proteins were identified in at least four LC-MS\/MS analyses out of eight. According to Reactome the most significant \u2018top three\u2019 1st level pathways for detected EVs-PMSC proteins were \u2018Cellular responses to external stimuli\u2019; \u2018Immune system\u2019; \u2018Developmental biology\u2019. Some of the most enriched pathways in the group \u2018Cellular responses to external stimuli\u2019 were: \u2018Response of EIF2AK4 (GCN2) to amino acid deficiency\u2019; \u2018Cellular response to hypoxia\u2019; \u2018Detoxification of Reactive Oxygen Species\u2019. Some of the most enriched pathways in the group \u2018Immune System\u2019 were: \u2018Class I MHC mediated antigen processing & presentation\u2019; \u2018Neutrophil degranulation\u2019; \u2018Interferon Signaling\u2019. The most enriched pathway in the group \u2018Developmental biology\u2019 was \u2018Axon guidance\u2019. Thus far, concerning most enriched pathways EVs-PMSC proteome is expected to: 1) provide protection against stress (amino acid deficiency, hypoxia, ROS) to the damaged tissues; 2) possess immunomodulation properties; 3) facilitate axon guidance. The result (the top list of enriched pathways) leads to the notion that EVs-PMSC could be effective in terms of neuroprotection.<\/p>\n","protected":false},"excerpt":{"rendered":"

Anastasia Khutornenko1, Anastasia Zharikova2, Vasily Popkov3, Sergey Kovalchuk4, Kirill Goryunov5, Yulia Shevtsova6, Egor Plotnikov7, Dmitry Zorov8, Denis Silachev91Laboratory of Cell Technologies, Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia, bioingenier@gmail.com2Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russia, azharikova89@gmail.com3The A.N. Belozersky Institute Of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia, popkov.vas@gmail.com4Laboratory of Bioinformatic methods for Combinatorial Chemistry and Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia, xerx222@gmail.com5Laboratory of Cell Technologies, Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia, kirishgor@gmail.com6Laboratory of Cell Technologies, Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia, yulshevtsova@yandex.ru7A.N. Belozersky Institute of Physico-Chemical Biology; Research Center of Obstetrics, Gynecology and Perinatology, Moscow, Russia, plotnikov@belozersky.msu.ru8A.N. Belozersky Institute of Physico-Chemical Biology; Reserch Center of Obstetrics, Gynecology and Perinatology, Moscow, Russia, zorov@belozersky.msu.ru9A.N. Belozersky Institute of Physico-Chemical Biology; Research Center of Obstetrics, Gynecology and Perinatology, Moscow, Russia, d_silachev@oparina4.ru Mesenchymal stem\/stromal cells (MSCs) have therapeutic potential in many pathological conditions, that is explained mostly by their paracrine action. MSCs secrete extracellular vesicles (EVs) packed with proteins, DNA, RNA and lipids. One of the easily accessible and reach sources of MSCs is placenta. In order to reveal possible pathways, by which EVs produced by placenta-derived MSCs (EVs-PMSC) could realize their therapeutic potential we conducted proteomic analysis of EVs-PMSC and subsequent bioinformatic analysis of identified proteins using Reactome pathway database. Identified peptides were mapped to 2093 proteins. Among the 2093 proteins, 972 proteins were identified in at least four LC-MS\/MS analyses out of eight. According to Reactome the most significant \u2018top three\u2019 1st level pathways for detected EVs-PMSC proteins were \u2018Cellular responses to external stimuli\u2019; \u2018Immune system\u2019; \u2018Developmental biology\u2019. Some of the most enriched pathways in the group \u2018Cellular responses to external stimuli\u2019 were: \u2018Response of EIF2AK4 (GCN2) to amino acid deficiency\u2019; \u2018Cellular response to hypoxia\u2019; \u2018Detoxification of Reactive Oxygen Species\u2019. Some of the most enriched pathways in the group \u2018Immune System\u2019 were: \u2018Class I MHC mediated antigen processing & presentation\u2019; \u2018Neutrophil degranulation\u2019; \u2018Interferon Signaling\u2019. The most enriched pathway in the group \u2018Developmental biology\u2019 was \u2018Axon guidance\u2019. Thus far, concerning most enriched pathways EVs-PMSC proteome is expected to: 1) provide protection against stress (amino acid deficiency, hypoxia, ROS) to the damaged tissues; 2) possess immunomodulation properties; 3) facilitate axon guidance. The result (the top list of enriched pathways) leads to the notion that EVs-PMSC could be effective in terms of neuroprotection.<\/p>\n","protected":false},"author":9,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[2165],"tags":[1789,1140,1833,2244,2245],"_links":{"self":[{"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/posts\/10148"}],"collection":[{"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/comments?post=10148"}],"version-history":[{"count":0,"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/posts\/10148\/revisions"}],"wp:attachment":[{"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/media?parent=10148"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/categories?post=10148"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/tags?post=10148"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}