{"id":10158,"date":"2020-06-29T09:36:52","date_gmt":"2020-06-29T09:36:52","guid":{"rendered":"https:\/\/bgrssb.icgbio.ru\/2020\/2020\/06\/29\/allelic-drop-out-is-a-common-phenomenon-reducing-the-diagnostic-yield-of-pcr-based-target-sequencing\/"},"modified":"2020-06-29T09:36:52","modified_gmt":"2020-06-29T09:36:52","slug":"allelic-drop-out-is-a-common-phenomenon-reducing-the-diagnostic-yield-of-pcr-based-target-sequencing","status":"publish","type":"post","link":"https:\/\/bgrssb.icgbio.ru\/2020\/2020\/06\/29\/allelic-drop-out-is-a-common-phenomenon-reducing-the-diagnostic-yield-of-pcr-based-target-sequencing\/","title":{"rendered":"Allelic drop-out is a common phenomenon reducing the diagnostic yield of PCR-based target sequencing"},"content":{"rendered":"

Poster (download<\/a>)
\n
276<\/a>
Anna Shestak1<\/sup>, Anna Bukaeva2<\/sup>, Siamak Saber3<\/sup>, Elena Zaklyazminskaya4<\/sup>
1<\/sup>Petrovsky National Research Center of Surgery, Moscow, Russia, anna.shestak87@gmail.com
2<\/sup>Petrovsky National Research Center of Surgery, Moscow, Russia, 16_anna_02@mail.ru
3<\/sup>Cardiac Electrophysiology Research Center, Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran, s_saber2002@yahoo.com
4<\/sup>Petrovsky National Research Center of Surgery, Moscow, Russia, helenezak@gmail.com<\/p>\n

Abstract<\/em><\/strong> <\/strong>Allelic drop-out (ADO) is a known phenomenon of selective allele amplification representing the potential problem of correct DNA diagnostics. The results demonstrate the incidence of ADO reducing the diagnostic yield of PCR-based target sequencing.\u0412\u00a0<\/strong><\/h5>\n

Motivation and aim:<\/strong> Allelic drop-out (ADO) is a known phenomenon of selective allele amplification representing the potential problem of correct DNA diagnostics. Both NGS and Sanger sequencing are PCR-based methods, Sanger sequencinq is used to verify NGS results. The aim of this study is to demonstrate the incidence of ADO reducing the diagnostic yield in primary cardiomyopathy genetic testing via semiconductor NGS and Sanger sequencing of target gene panels.<\/p>\n

Methods:<\/strong> We have developed 3 AmpliSeq custom gene panels for mutational screening: \u0432\u0402\u045aK+<\/sup>\/Na+<\/sup> ion channels\u0432\u0402\u045c, \u0432\u0402\u045aDesmosomal proteins\u0432\u0402\u045c, \u0432\u0402\u045aSarcomeric proteins\u0432\u0402\u045c, contains 1049 primer pairs (37 genes) totally, 152 kb. About 140 probands were screened with at least one of these gene panels. AmpliSeq sequences were analyzed in silico and visually compared with Sanger control sequences, noting the facts of heterozygosity loss.<\/p>\n

Results: <\/strong>We have detected 12 ADO cases both in Sanger (5 cases) and AmpliSeq (7 cases) sequencing data. All ADO events happened due to frequent or rare SNVs in the oligoprimer annealing sites and were detected due to mismatch in frequent SNPs zygosity nearby. Three pathogenic variants would be missed if were not revealed by re-sequencing with alternative method and alternative oligos.<\/p>\n

Conclusion: <\/strong>All PCR-based methods have a risk of ADO leading to a decrease of diagnostic yield of genetic testing.\u0412\u00a0ADO can theoretically affect 1% amplicons.\u0412\u00a0It seems that real scope of ADO might be much higher and depends on numbers of primer pairs. The software for ADO detection is needed.<\/p>\n

Acknowledgment:<\/strong> \u0412\u00a0This work was supported by Russian Science Foundation grant \u0432\u201e\u201316-15-10421.<\/p>\n","protected":false},"excerpt":{"rendered":"

Poster (download) Anna Shestak1, Anna Bukaeva2, Siamak Saber3, Elena Zaklyazminskaya41Petrovsky National Research Center of Surgery, Moscow, Russia, anna.shestak87@gmail.com2Petrovsky National Research Center of Surgery, Moscow, Russia, 16_anna_02@mail.ru3Cardiac Electrophysiology Research Center, Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran, s_saber2002@yahoo.com4Petrovsky National Research Center of Surgery, Moscow, Russia, helenezak@gmail.com Abstract Allelic drop-out (ADO) is a known phenomenon of selective allele amplification representing the potential problem of correct DNA diagnostics. The results demonstrate the incidence of ADO reducing the diagnostic yield of PCR-based target sequencing.\u0412\u00a0 Motivation and aim: Allelic drop-out (ADO) is a known phenomenon of selective allele amplification representing the potential problem of correct DNA diagnostics. Both NGS and Sanger sequencing are PCR-based methods, Sanger sequencinq is used to verify NGS results. The aim of this study is to demonstrate the incidence of ADO reducing the diagnostic yield in primary cardiomyopathy genetic testing via semiconductor NGS and Sanger sequencing of target gene panels. Methods: We have developed 3 AmpliSeq custom gene panels for mutational screening: \u0432\u0402\u045aK+\/Na+ ion channels\u0432\u0402\u045c, \u0432\u0402\u045aDesmosomal proteins\u0432\u0402\u045c, \u0432\u0402\u045aSarcomeric proteins\u0432\u0402\u045c, contains 1049 primer pairs (37 genes) totally, 152 kb. About 140 probands were screened with at least one of these gene panels. AmpliSeq sequences were analyzed in silico and visually compared with Sanger control sequences, noting the facts of heterozygosity loss. Results: We have detected 12 ADO cases both in Sanger (5 cases) and AmpliSeq (7 cases) sequencing data. All ADO events happened due to frequent or rare SNVs in the oligoprimer annealing sites and were detected due to mismatch in frequent SNPs zygosity nearby. Three pathogenic variants would be missed if were not revealed by re-sequencing with alternative method and alternative oligos. Conclusion: All PCR-based methods have a risk of ADO leading to a decrease of diagnostic yield of genetic testing.\u0412\u00a0ADO can theoretically affect 1% amplicons.\u0412\u00a0It seems that real scope of ADO might be much higher and depends on numbers of primer pairs. The software for ADO detection is needed. Acknowledgment: \u0412\u00a0This work was supported by Russian Science Foundation grant \u0432\u201e\u201316-15-10421.<\/p>\n","protected":false},"author":9,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[2171],"tags":[1714,1713,1715],"_links":{"self":[{"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/posts\/10158"}],"collection":[{"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/comments?post=10158"}],"version-history":[{"count":0,"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/posts\/10158\/revisions"}],"wp:attachment":[{"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/media?parent=10158"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/categories?post=10158"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/tags?post=10158"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}