{"id":10178,"date":"2020-06-29T09:36:52","date_gmt":"2020-06-29T09:36:52","guid":{"rendered":"https:\/\/bgrssb.icgbio.ru\/2020\/2020\/06\/29\/whole-genome-sequencing-and-assembly-of-saccharomyces-cerevisiae-genomes-using-oxford-nanopore-data\/"},"modified":"2020-06-29T09:36:52","modified_gmt":"2020-06-29T09:36:52","slug":"whole-genome-sequencing-and-assembly-of-saccharomyces-cerevisiae-genomes-using-oxford-nanopore-data","status":"publish","type":"post","link":"https:\/\/bgrssb.icgbio.ru\/2020\/2020\/06\/29\/whole-genome-sequencing-and-assembly-of-saccharomyces-cerevisiae-genomes-using-oxford-nanopore-data\/","title":{"rendered":"Whole genome sequencing and assembly of Saccharomyces cerevisiae genomes using Oxford Nanopore data"},"content":{"rendered":"<p>Andrew G. Matveenko<sup>1<\/sup>, Anton B. Matiiv<sup>2<\/sup>, Yury A. Barbitoff<sup>3<\/sup>, Evgenia M. Maksiutenko<sup>4<\/sup>, Svetlana E. Moskalenko<sup>5<\/sup>, Alexandra V. Beliavskaia<sup>6<\/sup>, Alexander V. Predeus<sup>7<\/sup>, Galina A. Zhouravleva<sup>8<\/sup><br \/><sup>1<\/sup>Dpt. of Genetics and Biotechnology, St. Petersburg State University, St. Petersburg, Russia, a.matveenko@spbu.ru<br \/><sup>2<\/sup>Dpt. of Genetics and Biotechnology, St. Petersburg State University, St. Petersburg, Russia, antonmatiiv@yandex.ru<br \/><sup>3<\/sup>Dpt. of Genetics and Biotechnology, St. Petersburg State University, St. Petersburg, Russia; Bioinformatics Institute, St. Petersburg, Russia, barbitoff@bk.ru<br \/><sup>4<\/sup>Vavilov Institute of General Genetics, St. Petersburg Branch, St. Petersburg, Russia; Dpt. of Genetics and Biotechnology, St. Petersburg State University, St. Petersburg, Russia, jmrose@yandex.ru<br \/><sup>5<\/sup>Vavilov Institute of General Genetics, St. Petersburg Branch, St. Petersburg, Russia; Dpt. of Genetics and Biotechnology, St. Petersburg State University, St. Petersburg, Russia, s.moskalenko@spbu.ru<br \/><sup>6<\/sup>University of Liverpool, Liverpool, UK, alex.beliavskaia@gmail.com<br \/><sup>7<\/sup>University of Liverpool, Liverpool, UK; Bioinformatics Institute, St. Petersburg, Russia, predeus@bioinf.me<br \/><sup>8<\/sup>Dpt. of Genetics and Biotechnology, St. Petersburg State University, St. Petersburg, Russia, g.zhuravleva@spbu.ru<\/p>\n<p>The Peterhof genetic collection (PGC) of yeast\u0412\u00a0<em><i>S<\/i><\/em><em><i>accharomyces<\/i><\/em><em><i>\u0412\u00a0cerevisiae <\/i><\/em>is one of the rare\u0412\u00a0examples\u0412\u00a0of a large genetic collection established independently of reference S288C strain. We assembled genomes of two widely used PGC strains, 1A-D1628 and 74-D694, using Oxford Nanopore MinION sequencing data. Subsequent analysis of structural variations showed a number of differences between PGC strains and S288C.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Andrew G. Matveenko1, Anton B. Matiiv2, Yury A. Barbitoff3, Evgenia M. Maksiutenko4, Svetlana E. Moskalenko5, Alexandra V. Beliavskaia6, Alexander V. Predeus7, Galina A. Zhouravleva81Dpt. of Genetics and Biotechnology, St. Petersburg State University, St. Petersburg, Russia, a.matveenko@spbu.ru2Dpt. of Genetics and Biotechnology, St. Petersburg State University, St. Petersburg, Russia, antonmatiiv@yandex.ru3Dpt. of Genetics and Biotechnology, St. Petersburg State University, St. Petersburg, Russia; Bioinformatics Institute, St. Petersburg, Russia, barbitoff@bk.ru4Vavilov Institute of General Genetics, St. Petersburg Branch, St. Petersburg, Russia; Dpt. of Genetics and Biotechnology, St. Petersburg State University, St. Petersburg, Russia, jmrose@yandex.ru5Vavilov Institute of General Genetics, St. Petersburg Branch, St. Petersburg, Russia; Dpt. of Genetics and Biotechnology, St. Petersburg State University, St. Petersburg, Russia, s.moskalenko@spbu.ru6University of Liverpool, Liverpool, UK, alex.beliavskaia@gmail.com7University of Liverpool, Liverpool, UK; Bioinformatics Institute, St. Petersburg, Russia, predeus@bioinf.me8Dpt. of Genetics and Biotechnology, St. Petersburg State University, St. Petersburg, Russia, g.zhuravleva@spbu.ru The Peterhof genetic collection (PGC) of yeast\u0412\u00a0Saccharomyces\u0412\u00a0cerevisiae is one of the rare\u0412\u00a0examples\u0412\u00a0of a large genetic collection established independently of reference S288C strain. We assembled genomes of two widely used PGC strains, 1A-D1628 and 74-D694, using Oxford Nanopore MinION sequencing data. Subsequent analysis of structural variations showed a number of differences between PGC strains and S288C.<\/p>\n","protected":false},"author":9,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[2171],"tags":[1648,1785,1784,1786],"_links":{"self":[{"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/posts\/10178"}],"collection":[{"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/comments?post=10178"}],"version-history":[{"count":0,"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/posts\/10178\/revisions"}],"wp:attachment":[{"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/media?parent=10178"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/categories?post=10178"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2020\/wp-json\/wp\/v2\/tags?post=10178"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}