by Anna Pelevina | J.J. Berestovskaya | A.G. Dorofeev | N.V. Ravin | A.V.Mardanov | N.V. Pimenov |
Winogradsky Institute of Microbiology, Research Center of Biotechnology RAS | Winogradsky Institute of
Microbiology, Research Center of Biotechnology RAS | Winogradsky Institute of Microbiology, Research
Center of Biotechnology RAS | Skryabin Institute of Bioengineering, Research Center of Biotechnology
RAS | Skryabin Institute of Bioengineering, Research Center of Biotechnology RAS | Winogradsky
Institute of Microbiology, Research Center of Biotechnology RAS
A microbial community enriched with phosphate-accumulating organisms (PAO) was
obtained in a laboratory sequencing batch reactor after 150 days of cultivation. Analysis of
phosphate dynamics in the medium and the results of Raman scattering spectroscopy
indicated the cycle of phosphate consumption and release during cultivation under oxic and
anoxic conditions, respectively, which was typical of PAO. The highest content of
intracellular phosphorus was 16.5 ± 0.15% of the dry ash-free biomass. Molecular genetic
analysis and FISH revealed the taxonomic diversity of the microbial community, in which
members of the “Ca. Аccumulibacter” group were the major PAO.