{"id":15604,"date":"2022-06-21T11:05:48","date_gmt":"2022-06-21T04:05:48","guid":{"rendered":"https:\/\/bgrssb.icgbio.ru\/2022\/?p=15604"},"modified":"2022-09-20T10:32:56","modified_gmt":"2022-09-20T03:32:56","slug":"melatonin-effect-on-the-thymus-cell-composition-in-mice-with-functional-pinealectomy","status":"publish","type":"post","link":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/2022\/06\/21\/melatonin-effect-on-the-thymus-cell-composition-in-mice-with-functional-pinealectomy\/","title":{"rendered":"Melatonin effect on the thymus cell composition in mice with functional pinealectomy"},"content":{"rendered":"

by Michurina S.V. | Miroshnichenko S.M. | Ishchenko I.Yu. | Serykh A.E. | Rachkovskaya L.N.1 |<\/em>
\n1Research Institute of Clinical and Experimental Lymphology — Branch of the Institute of Cytology and<\/em>
\nGenetics, SB RAS, Novosibirsk, Russia | 1Research Institute of Clinical and Experimental Lymphology —<\/em>
\nBranch of the Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia 2Research Institute of<\/em>
\nBiochemistry \u2013 Federal Research Center for Fundamental and Translational Medicine, Novosibirsk,<\/em>
\nRussia | 1Research Institute of Clinical and Experimental Lymphology — Branch of the Institute of<\/em>
\nCytology and Genetics, SB RAS, Novosibirsk, Russia | 1Research Institute of Clinical and Experimental<\/em>
\nLymphology — Branch of the Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia3Research<\/em>
\nInstitute of Experimental and Clinical Medicine — Federal Research Center for Fundamental and<\/em>
\nTranslational Medicine, Novosibirsk, Russia | Institute of Clinical and Experimental Lymphology — a<\/em>
\nbranch of Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia;<\/em><\/p>\n

Motivation and Aim<\/strong>: Prolonged continuous illumination contributes to the development of
\nfunctional pinealectomy, a decrease in adaptive cellular and humoral immune response. The
\nstudy aim was to investigate the melatonin effect on the content of young CD3low and
\nmature CD3hi T-lymphocyte forms in the thymus of C57Bl\/6j mice with a model of functional
\npinealectomy (FP).
\nMethods and Algorithms<\/strong>: Four animal groups were formed. «CL» \u2013 mice with the FP model,
\nwhich was created by keeping animals under continuous lighting (CL) for 14 days
\n(photoperiod light:dark 24:0 hours). «CL+MT» — animals with FP which were intragastrically
\ninjected with melatonin (MT, 0.664 g\/kg body weight) in 200\u03bcl of distilled water daily
\nagainst a background of CL. «CL+Placebo» — mice with FP which received water 200\u03bcl
\nintragastrically daily. «Control» — intact mice kept under standard lighting mode (14:10 h).
\nImmunophenotyping of CD3hi and CD3low thymocyte subpopulations was performed by
\nstaining using CD3-ARS antibodies and analyzed using a flow cytofluorimeter. Statistical
\nanalysis was carried out using Mann-Whitney test («Statistica 12»).
\nResults<\/strong>: In FP mice, both a decrease in the relative number of CD3low and CD3hi
\nthymocytes and a significant decline in the CD3low\/CD3hi ratio were found. Treatment of
\nanimals with melatonin under CL restored the percentage of CD3low and CD3hi thymocytes,
\nas well as the CD3low\/CD3hi ratio to control values.
\nConclusion<\/strong>: Thus the results indicate that prolonged continuous illumination has a
\ndepressing effect on differentiation and maturation of young thymocytes into mature forms.
\nMelatonin treatment in this model situation helps to compensate for the CL effects by
\ntriggering the process of cell proliferation in the thymus from the earliest stages. This
\nindicates that melatonin has immunotropic properties and can be used to correct the consequences of functional pinealectomy.<\/p>\n\u041f\u043e\u0441\u0442\u0435\u0440-Michurina-S.V.-Miroshnichenko-S.M.-Ishchenko-I.-Yu.-et-al.<\/a>\n","protected":false},"excerpt":{"rendered":"

by Michurina S.V. | Miroshnichenko S.M. | Ishchenko I.Yu. | Serykh A.E. | Rachkovskaya L.N.1 | 1Research Institute of Clinical and Experimental Lymphology — Branch of the Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia | 1Research Institute of Clinical and Experimental Lymphology — Branch of the Institute of Cytology and Genetics, SB RAS, […]<\/p>\n","protected":false},"author":3967,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[12],"tags":[54,55,56,57,58,59],"_links":{"self":[{"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/posts\/15604"}],"collection":[{"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/users\/3967"}],"replies":[{"embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/comments?post=15604"}],"version-history":[{"count":3,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/posts\/15604\/revisions"}],"predecessor-version":[{"id":15871,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/posts\/15604\/revisions\/15871"}],"wp:attachment":[{"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/media?parent=15604"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/categories?post=15604"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/tags?post=15604"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}