{"id":15618,"date":"2022-06-21T11:19:59","date_gmt":"2022-06-21T04:19:59","guid":{"rendered":"https:\/\/bgrssb.icgbio.ru\/2022\/?p=15618"},"modified":"2022-09-20T10:32:56","modified_gmt":"2022-09-20T03:32:56","slug":"the-new-general-biological-property-of-stem-like-tumor-cells","status":"publish","type":"post","link":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/2022\/06\/21\/the-new-general-biological-property-of-stem-like-tumor-cells\/","title":{"rendered":"The new general biological property of stem-like tumor cells"},"content":{"rendered":"<p><em>by Evgeniya V. Dolgova | Institute of Cytology and Genetics SB RAS<\/em><\/p>\n<p><strong>Motivation and Aim<\/strong>: Previously, we have demonstrated poorly differentiated cells of various<br \/>\norigin and genesis, including tumor stem-like ones, to be capable of native, i.e. without<br \/>\nusing any artificial compounds or procedures, internalizing extracellular double-stranded<br \/>\nDNA (dsDNA) fragments. However, the exact mechanism of extracellular dsDNA<br \/>\ninternalization into cells has not yet been clarified.<br \/>\n<strong>Methods and Algorithms<\/strong>: In the current report, for murine Krebs-2 carcinoma and human<br \/>\nEBV+ B-lymphoma, there have been determined the mechanisms mediating the interaction<br \/>\nof dsDNA with the surface of tumor stem-like cells (TSCs) and its consequent internalization<br \/>\ninto these cells. The following approaches were used: electrophoretic separation of cells in a<br \/>\nfree volume or pre-exposure of cells to various proteases and\/or compounds inhibiting pinoand endocytosis, followed by assessing the resulting efficiency of Alu-TAMRA DNA probe<br \/>\ninternalization; analysis of the transcriptome of the indicated cell lines followed by the qPCR<br \/>\nto validate the differences in expression of some target genes identified as possible<br \/>\nparticipants in the process of dsDNA internalization.<br \/>\n<strong>Results<\/strong>: It was shown, that the process of binding and internalizing is rather complicated<br \/>\nand composed of the following successive stages: (i) initiating electrostatic interaction and<br \/>\ncontact of a negatively charged dsDNA molecule with a positively charged molecule(s) on<br \/>\nthe surface of a TSC; (ii) binding of the dsDNA probe to a tumor stem cell surface protein(s)<br \/>\nvia the formation of a strong chemical\/molecular bond; (iii) the very internalization of<br \/>\ndsDNA into the cell. Inhibitors of caveolae-dependent internalization abrogate the DNA<br \/>\nuptake in Krebs-2 cells, and inhibitors of clathrin\/caveolar mechanism block the<br \/>\ninternalization in EBV+ B-lymphoma cells.<br \/>\n<strong>Conclusion<\/strong>: The basic provisions of the concept characterizing the principle of the<br \/>\norganization of the stem-like tumor cell surface factors to be formulated as a concept of<br \/>\n\u201cgroup-specific cell surface factors, which form the profile of tumor stem-like cells\u201d.<\/p>\n<a href=\"https:\/\/bgrssb.icgbio.ru\/2022\/wp-content\/uploads\/sites\/3\/2022\/06\/Poster_Dolgova.pdf\" class=\"pdfemb-viewer\" style=\"\" data-width=\"max\" data-height=\"max\"  data-toolbar=\"bottom\" data-toolbar-fixed=\"off\">Poster_Dolgova<br\/><\/a>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>by Evgeniya V. Dolgova | Institute of Cytology and Genetics SB RAS Motivation and Aim: Previously, we have demonstrated poorly differentiated cells of various origin and genesis, including tumor stem-like ones, to be capable of native, i.e. without using any artificial compounds or procedures, internalizing extracellular double-stranded DNA (dsDNA) fragments. However, the exact mechanism of [&hellip;]<\/p>\n","protected":false},"author":3967,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[15],"tags":[68,65,69,66,70,67,71,72],"_links":{"self":[{"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/posts\/15618"}],"collection":[{"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/users\/3967"}],"replies":[{"embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/comments?post=15618"}],"version-history":[{"count":1,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/posts\/15618\/revisions"}],"predecessor-version":[{"id":15620,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/posts\/15618\/revisions\/15620"}],"wp:attachment":[{"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/media?parent=15618"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/categories?post=15618"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/tags?post=15618"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}