{"id":16074,"date":"2022-07-04T22:24:05","date_gmt":"2022-07-04T15:24:05","guid":{"rendered":"https:\/\/bgrssb.icgbio.ru\/2022\/?p=16074"},"modified":"2022-09-20T10:31:55","modified_gmt":"2022-09-20T03:31:55","slug":"evaluation-of-the-bacterial-ribosomal-protein-s1-gene-rpsa-from-the-position-of-structural-repetition","status":"publish","type":"post","link":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/2022\/07\/04\/evaluation-of-the-bacterial-ribosomal-protein-s1-gene-rpsa-from-the-position-of-structural-repetition\/","title":{"rendered":"Evaluation of the bacterial ribosomal protein S1 gene (rpsA) from the position of structural repetition"},"content":{"rendered":"<p><em>by Machulin A | Deryusheva E. | Skryabin Institute of Biochemistry and Physiology of Microorganisms,<\/em><br \/>\n<em>Federal Research Center \u201cPushchino Scientific Center for Biological Research of the Russian Academy of<\/em><br \/>\n<em>Sciences\u201d, Pushchino , Russia | Institute for Biological Instrumentation, Federal Research Center<\/em><br \/>\n<em>\u201cPushchino Scientific Center for Biological Research of the Russian Academy of Sciences\u201d, Pushchino,<\/em><br \/>\n<em>Russia<\/em><\/p>\n<p>The family of bacterial ribosomal S1 proteins (rpsA) with repetitive S1 domains differs in a<br \/>\ncanonical sense from other similar proteins. The S1 protein is essential for cell viability<br \/>\nbecuase it interacts with both mRNA and proteins. Protein domain repeats are known to<br \/>\narise due to tandem duplications of internal genes. To study the mechanisms of repeat<br \/>\nexpansion, we studied 1324 rpsA sequences of S1 proteins with different number of S1<br \/>\ndomains. Analysis of rpsA showed that the gene regions encoding individual S1 domains<br \/>\nhave no a strictly repetitive structure between groups containing different number of<br \/>\ndomains. The part of rpsA encoding the central domains in multidomain S1 proteins is more<br \/>\nconserved than the terminal domains, which correlates with the fact that duplication is<br \/>\nfound predominantly in the central region of the protein chain between other repeats. The<br \/>\nmaximum value of rpsA identity for full-length proteins was found for S1 proteins containing<br \/>\nsix structural domains (58%). The predominance of four- and six-domain S1 proteins can be<br \/>\nexplained by the fact that the formation of repeating regions is based on the duplication of<br \/>\nseveral domains, while duplication of one domain is rare. The regionss of rpsA genes<br \/>\nencoding adjacent domains are more identical, which is consistent with the data suggesting<br \/>\nthat duplication occurs predominantly in the middle of the protein chain between other<br \/>\nrepeats. The regions of the gene encoding residues that form the RNA-binding site remain<br \/>\nconserved. All the results obtained characterize the structural features of rpsA that<br \/>\ndetermine the functioning of the S1 proteins.<\/p>\n<a href=\"https:\/\/bgrssb.icgbio.ru\/2022\/wp-content\/uploads\/sites\/3\/2022\/07\/BGRSSB-2022_S1.pdf\" class=\"pdfemb-viewer\" style=\"\" data-width=\"max\" data-height=\"max\"  data-toolbar=\"bottom\" data-toolbar-fixed=\"off\">BGRSSB-2022_S1<br\/><\/a>\n","protected":false},"excerpt":{"rendered":"<p>by Machulin A | Deryusheva E. | Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center \u201cPushchino Scientific Center for Biological Research of the Russian Academy of Sciences\u201d, Pushchino , Russia | Institute for Biological Instrumentation, Federal Research Center \u201cPushchino Scientific Center for Biological Research of the Russian Academy of Sciences\u201d, Pushchino, Russia [&hellip;]<\/p>\n","protected":false},"author":3967,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5],"tags":[368,369,370,367,371],"_links":{"self":[{"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/posts\/16074"}],"collection":[{"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/users\/3967"}],"replies":[{"embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/comments?post=16074"}],"version-history":[{"count":1,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/posts\/16074\/revisions"}],"predecessor-version":[{"id":16076,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/posts\/16074\/revisions\/16076"}],"wp:attachment":[{"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/media?parent=16074"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/categories?post=16074"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/ru\/wp-json\/wp\/v2\/tags?post=16074"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}