{"id":15621,"date":"2022-06-21T11:21:36","date_gmt":"2022-06-21T04:21:36","guid":{"rendered":"https:\/\/bgrssb.icgbio.ru\/2022\/?p=15621"},"modified":"2022-09-20T10:32:56","modified_gmt":"2022-09-20T03:32:56","slug":"the-new-method-of-genetic-barcoding","status":"publish","type":"post","link":"https:\/\/bgrssb.icgbio.ru\/2022\/2022\/06\/21\/the-new-method-of-genetic-barcoding\/","title":{"rendered":"The new method of genetic barcoding"},"content":{"rendered":"<p><em>by Olga Kiryanova | Irek Gubaydullin | Alexey Chemeris | Ufa State Petroleum Technological University |<\/em><br \/>\n<em>Institute of Petrochemistry and Catalysis, Ufa Federal Research Center | Institute of Biochemistry and<\/em><br \/>\n<em>Genetics, Ufa Federal Research<\/em><\/p>\n<p><strong>Motivation and Aim<\/strong>: The classification and systematization of living organisms is currently<br \/>\nan actual task. The cytochrome oxidase gene was assumed as a reference region for the<br \/>\nliving organisms. Here we propose a new method for DNA certification of living organisms<br \/>\non the example of plants.<br \/>\n<strong>Methods and Algorithms<\/strong>: We have utilized in silico multiplex PCR analysis. Namely, we<br \/>\nhave found the assumed primer annealing sites in a genome using the Knuth\u2013Morris\u2013Pratt<br \/>\nalgorithm. The amplicons corresponding to the annealing sites were converted into a binary<br \/>\nformat. The binary sequence is easy enough to translate into a barcode format.<br \/>\n<strong>Results<\/strong>: We have conducted a comparative analysis of the following plant genomes: wheat<br \/>\n(6 genomes), hevea (3 genomes), and arabidopsis (9 genomes). The proposed approach was<br \/>\nutilized to obtain barcodes for each group. Each set of barcodes indicated the differences<br \/>\nand similarities for certain plants. It should be pointed out, that different primers provide<br \/>\ndifferent barcodes for the same genome. In this case, computer analysis makes it possible to<br \/>\nidentify successful primers for the experimental PCR.<br \/>\n<strong>Conclusion<\/strong>: The presented DNA certification can reveal similarities and differences at the<br \/>\nlevel of large order taxa (species, family) however it more reliable for the certification of<br \/>\ncultivars. Hence, it allows to test several primers as well as to get an idea of the success<br \/>\nexperiment. Thus, the proposed method provides a reliable and convenient way to store<br \/>\ngenetic data in a digital format, and reduce the time consumption in PCR tests planning.<br \/>\n<strong>Acknowledgements<\/strong>: The study was funded by Russian Foundation for Basic Research<br \/>\n(17-44-020120).<\/p>\n<a href=\"https:\/\/bgrssb.icgbio.ru\/2022\/wp-content\/uploads\/sites\/3\/2022\/06\/Kiryanova_presentation_.pdf\" class=\"pdfemb-viewer\" style=\"\" data-width=\"max\" data-height=\"max\"  data-toolbar=\"bottom\" data-toolbar-fixed=\"off\">Kiryanova_presentation_<br\/><\/a>\n","protected":false},"excerpt":{"rendered":"<p>by Olga Kiryanova | Irek Gubaydullin | Alexey Chemeris | Ufa State Petroleum Technological University | Institute of Petrochemistry and Catalysis, Ufa Federal Research Center | Institute of Biochemistry and Genetics, Ufa Federal Research Motivation and Aim: The classification and systematization of living organisms is currently an actual task. The cytochrome oxidase gene was assumed [&hellip;]<\/p>\n","protected":false},"author":3967,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[10],"tags":[73,74,75],"_links":{"self":[{"href":"https:\/\/bgrssb.icgbio.ru\/2022\/wp-json\/wp\/v2\/posts\/15621"}],"collection":[{"href":"https:\/\/bgrssb.icgbio.ru\/2022\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/bgrssb.icgbio.ru\/2022\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/wp-json\/wp\/v2\/users\/3967"}],"replies":[{"embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/wp-json\/wp\/v2\/comments?post=15621"}],"version-history":[{"count":1,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/wp-json\/wp\/v2\/posts\/15621\/revisions"}],"predecessor-version":[{"id":15623,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/wp-json\/wp\/v2\/posts\/15621\/revisions\/15623"}],"wp:attachment":[{"href":"https:\/\/bgrssb.icgbio.ru\/2022\/wp-json\/wp\/v2\/media?parent=15621"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/wp-json\/wp\/v2\/categories?post=15621"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/bgrssb.icgbio.ru\/2022\/wp-json\/wp\/v2\/tags?post=15621"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}