Poster (download)
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Alexandr Poveshchenko1
1Research Institute of Clinical andExperimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences Novosibirsk, Russia, poveshchenkoa200@mail.ru
Breast cancer was induced by the administration of n-methyl-N-nitrosourea to Wistar rats. Some animals underwent only surgical intervention or only polychemotherapy (cyclophosphamide, methotrexate, 5-ftorouracil). In some animals, both types of therapy were combined, and in a separate group, the administration of the Panagen drug, which was a fragmented DNA molecule, was added to polychemotherapy. To study the concentration of cytokines in lymph, we used the Bio-Plex Pro Rat Cytokoness 24-Plex Assay test system (Bio-Rad, USA). It was shown that in rats with breast cancer the content in the lymph and blood of most of the studied cytokines such as IL-1ОІ, IL-2, IL-4, IL-6, IL-7, IL-12, IL-13, MIP-1О±, MIP-3О±, RANTES, TNF-О±, MCP-1 were statistically significantly higher, and IL-10 and GRO / KC, lower than in intact animals. Surgical removal of the tumor led to a significant decrease in the content of both pro-inflammatory and anti-inflammatory cytokines in the lymph. PCT led to a significant decrease in the content of IL-1ОІ, IL-4, IL-6, IL-7, MIP-1О±, MIP-3О±, RANTES in the blood serum and lymph of rats with breast cancer.