Identification of an AP2/ERF Transcription Factor Controlling the Synthesis of Barley Epicuticular Wax

Poster (download)

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Ekaterina Kolosovskaya¹, Sophia Gerasimova², Anna Korotkova³, Christian Hertig⁴, Sergey Morozov⁵, Elena Chernyak⁶, Dmitriy Domrachev⁷, Vikhorev Alexander⁸, Nikolay Shmakov⁹, Alexey Kochetov¹⁰, Jochen Kumlehn¹¹, Elena Khlestkina¹²
¹ICG SB RAS, Novosibirsk, Russia, kolosovskaya@bionet.nsc.ru
²ICG SB RAS, Novosibirsk, Russia NSU, Novosibirsk, Russia, gerson@bionet.nsc.ru
³ICG SB RAS, Novosibirsk, Russia, korotkova@bionet.nsc.ru
⁴Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Germany, hertig@ipk-gatersleben.de
⁵Novosibirsk Institute of Organic Chemistry, SB RAS Novosibirsk, Russia, moroz@nioch.nsc.ru
⁶Novosibirsk Institute of Organic Chemistry, SB RAS Novosibirsk, Russia, chernyak@nioch.nsc.ru
⁷Novosibirsk Institute of Organic Chemistry, SB RAS Novosibirsk, Russia, dmitry@nioch.nsc.ru
⁸ICG SB RAS, Novosibirsk, Russia NSU, Novosibirsk, Russia, vikhorev@bionet.nsc.ru
⁹ICG SB RAS, Novosibirsk, Russia, shmakov@bionet.nsc.ru
¹⁰ICG SB RAS, Novosibirsk, Russia NSU, Novosibirsk, Russia, ak@bionet.nsc.ru
¹¹Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Germany, kumlehn@ipk-gatersleben.de
¹²ICG SB RAS, Novosibirsk, Russia NSU, Novosibirsk, Russia Vavilov Institute of Plant Genetic Resources (VIR) Saint Petersburg, Russia, khlest@bionet.nsc.ru

Site-directed mutagenesis provides ample opportunity for reverse genetics, which allows us to establish a relationship between a gene and its function. In the present study, the role of the HvWIN1 barley (Hordeum vulgare) gene in the formation of epicuticular wax was identified by taking a targeted knockout approach using RNA-guided Cas9 endonuclease technology.