Poster (download)
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Darya V. Spiryova1, Alexander E. Moskalensky2, Alexei Vorob’ev3
1NSU, gerd.raz@yandex.ru
2NSU, a.mosk@nsu.ru
3NSU, vor@nioch.nsc.ru
Activation of blood platelets is the main process in normal hemostasis. Unfortunately, it also makes a great contribution to the development of cardiovascular diseases. In this work, we study activation dynamics using cytosolic calcium probe. To increase the accuracy of dynamic measurements, we present a method for optical activation of single platelets in suspension. It is achieved by the use of photolabile “caged” activation agonists. Futhermore, a mathematical model was developed that allows to detect individual calcium peaks and derive their dynamic parameters.