International Multiconference “Bioinformatics of Genome Regulation and Structure\Systems Biology”

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Systems Biology and Biomedicine symposium

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Systems Biology and Biomedicine symposiumContinuous glucose monitoring parameters in insulin-treated type 2 diabetic patients: relationships with obesity and body composition

Continuous glucose monitoring parameters in insulin-treated type 2 diabetic patients: relationships with obesity and body composition

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Poster (download)

[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/457.pdf”]
Julia F. Semenova1, Olga N. Fazullina2
1Laboratory of Endocrinology Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences(RICEL – Branch of IC&G SB RAS) Novosibirsk, Russia, ekmxtyjr@yandex.ru
2Laboratory of Endocrinology Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (RICEL – Branch of IC&G SB RAS) Novosibirsk, Russia, fazullina@ngs.ru

Background and aim: Obesity and associated insulin resistance can modify daily blood glucose fluctuations in patients with diabetes. The aim of our study was to determine the relationships between obesity, body composition and daily dynamics of glucose, assessed by continuous glucose monitoring (CGM), in patients with type 2 diabetes treated with insulin.
Materials and Methods: One hundred and thirty six insulin-treated patients with type 2 diabetes were examined. Real-time or blinded CGM was performed using Medtronic CGM devices in hospital settings. Time in ranges and a panel of GV parameters were derived from CGM recordings.
Results: Patients with obesity, as compared to those without, demonstrated significantly reduced Time Below Range (p<0.001), Low Blood Glucose Index (p<0.001), Lability Index (p=0.04), Men Absolute Glucose (p=0.03) and a tendency to lower Mean Amplidude of Glucose Excursions (p=0.08) and higher glycated hemoglobin HbA1c (p=0.07). There were negative correlations between total fat mass and Standard Deviation (r=-0.53, p=0.0008), Mean Amplitude of Glucose Excursions (r=-0.38, p=0.02), Lability Index (r=-0.51, p=0.0008), High Blood Glucose Index (r=-0.33, p=0.04) and MAG (r=-0.57, p=0.0001). Truncal fat mass, android and gynoid fat mass demonstrated negative correlations with these GV parameters also.
Conclusions: These data demonstrate the association between obesity, body composition and CGM parameters in insulin-treated type 2 diabetic subjects. The presence of obesity and accumulation of adipose tissue is associated with reduced GV and diminished risk of hypoglycemia.

Systems Biology and Biomedicine symposiumAccumulation of oxidative hepatobiliary lesions during experimental opisthorchiasis

Accumulation of oxidative hepatobiliary lesions during experimental opisthorchiasis

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Oxana Zaparina1, Anna Kovner2, Maria Pakharukova3, Viacheslav Mordvinov4
1ICG SB RAS, zp.oksana.93@gmail.com
2ICG SB RAS, kovner@bionet.nsc.ru
3ICG SB RAS, NSU, pakharukova@bionet.nsc.ru
4ICG SB RAS, mordvin@bionet.nsc.ru

Opisthorchiasis caused by the liver fluke Opisthorchis felineus in the hepatobiliary system of humans and fish-eating mammals is widespread on Russian territory. Pathogenesis of opisthorchiasis is accompanied by structural and functional disorders of the liver, including the formation of foci of chronic inflammation, cholestasis, cholecystitis and precancerous changes in the epithelium of the bile ducts.

In the result of study, we showed time- dependent oxidative lesions accumulation and hepatoprotective effect of various antioxidants

 

Systems Biology and Biomedicine symposiumHow miRNAs can protect humans from coronaviruses COVID-19, SARS-CoV, and MERS-CoV

How miRNAs can protect humans from coronaviruses COVID-19, SARS-CoV, and MERS-CoV

No Comment on How miRNAs can protect humans from coronaviruses COVID-19, SARS-CoV, and MERS-CoV

Anatoliy Ivashchenko1, Aizhan Rakhmetullina2, Aigul Akimniyazova3, Dana Aisina4
1Al-Farabi Kazakh National University, a.iavashchenko@gmail.com
2Al-Farabi Kazakh National University, zhanullina1994@gmail.com
3Al-Farabi Kazakh National University, akimniyazova@gmail.com
4Al-Farabi Kazakh National University, dana.aisina03@gmail.com

Viral diseases cause significant harm to human health and often cause high mortality. In the past twenty years, humanity has undergone infection by SARS-CoV (severe acute respiratory syndrome), MERS-CoV (Middle East respiratory syndrome) and COVID-19 coronaviruses, which spread from animals to humans and from person to person. These diseases have led to large economic losses. To fight coronaviruses and other viruses, it is proposed to use miRNAs, which regulate protein synthesis at the translational level. Out of 2565 miRNAs, miR-4778-3p, miR-6864-5p and miR-5197-3p were identified as the most effectively interacting with the gRNA of SARS-CoV, MERS-CoV and COVID-19, respectively. Based on the miR-4778-3p, miR-6864-5p and miR-5197-3p sequences, complete complementary miRNA (cc-miR) binding sites in the gRNA coronaviruses were created. Detected binding sites of these cc-miRs did not form intramolecular complexes in the 2D structure of the gRNA of SARS-CoV, MERS-CoV, and COVID-19 with a value of more than 85%. Therefore, cc-miRs will bind gRNA at these sites without competition. The cc-miRs for SARS-CoV, MERS-CoV, and COVID-19 did not have target genes among the 17508 human coding genes with a О”G/О”Gm of more than 85%, which implies the absence of side effects of cc-miRs on the translation of human mRNAs. cc-miRs can be used as therapeutic agents by incorporating them into exosomes or other vesicles and introducing them into the blood or lung by inhalation. The introduction of cc-miR into the blood will suppress the reproduction of the virus in the blood and in all organs into which it can enter.

Systems Biology and Biomedicine symposiumCatalytically active bispecific antibodies – new biochemical markers of HIV/AIDS

Catalytically active bispecific antibodies – new biochemical markers of HIV/AIDS

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Svetlana Baranova1, Sergey Sedykh2, Georgy Nevinsky3
1SB RAS ICBFM, swb@niboch.nsc.ru
2SB RAS ICBFM, NSU, sedyh@niboch.nsc.ru
3SB RAS ICBFM, NSU, nevinsky@niboch.nsc.ru

HIV-infection is a viral disease, causing an immune deficiency. In patients with HIV/AIDS there were described a number of changes similar to autoimmune processes.В An important problem of HIV/AIDS research is the prognosis of infection. As it was previously shown, autoimmune processes damage the nervous systems, and one of the markers of these processes are catalytic antibodies and bispecific antibodies. Such antibodies in vitroВ can hydrolyze neurospecific substrates.

Similiar antibodies were described in the blood of healthy donors, patients with systemic lupus erythematosus (SLE) and multiple sclerosis (MS). Proteolytic IgGs hydrolyze myelin basic protein and oligodendrocytic peptide. We have shown that sera of HIV/AIDS, SLE and MS patients contain autoantibodies against histones and myelin basic protein.

Here we show the results of research of natural bispecific catalytically active antibodies isolated from the blood of HIV/AIDS patients. Also, we compared the level of the protease activity of antibodies with the stage and characteristics of the pathological process. Interestingly, the pathological processes in HIV/AIDS are similar with such in MS and SLE. The development of new methods for diagnosing the patient’s condition, the effectiveness of therapy, and also predicting disease outcome in the future can be used for personalized therapy and improving the patient’s quality of life.

The research was supported by Grant of RFBR в„– 20-34-70115.

Systems Biology and Biomedicine symposiumPossible effect of SNP TATA-boxes of human erythropoiesis gene promoters on cognitive disorders

Possible effect of SNP TATA-boxes of human erythropoiesis gene promoters on cognitive disorders

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Poster (download)

[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/444.pdf”]
Ekaterina Sharypov1, Irina Drachkova2, Irina Chadaeva3, Mikhail Ponomarenko4, Ludmila Savinkova5
1Institute of Cytology and Genetics of SB RAS, sharypova@bionet.nsc.ru
2Institute of Cytology and Genetics of SB RAS, drachkova@bionet.nsc.ru
3Institute of Cytology and Genetics of SB RAS, ichadaeva@bionet.nsc.ru
4Institute of Cytology and Genetics of SB RAS, pon@bionen.nsc.ru
5Institute of Cytology and Genetics of SB RAS, lksav@bionet.nsc.ru

In recent years, there is increasing evidence that various forms of anemia, changes in the quantity and quality of blood cells, may be involved in the pathogenesis of various cognitive disorders accompanying Alzheimer\’s and Parkinson’s diseases, depression of various degrees, etc. In addition to erythroid cells, hemoglobin has been shown to be widely expressed in non-erythroid cells, including neurons of different parts of the brain.В  We analyzed in silico and in vitro unannotated SNPs in TATA boxes of human genes involved in erythropoiesis. Experimental verification in vitro using the method of electrophoretic mobility shift assay (EMSA) showed the correspondence of prognosis and experimental data. The obtained estimates of the effect of TATA box SNP markers on the formation of TBP/TATA complexes make it possible to consider some SNP markers of erythroid genes as markers of cognitive disorders.

Systems Biology and Biomedicine symposiumCytokines of the IL-6 family in the modulation of carbohydrate and lipid metabolism in patients with metabolic syndrome

Cytokines of the IL-6 family in the modulation of carbohydrate and lipid metabolism in patients with metabolic syndrome

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Daria Skuratovskaia1, Alexandra Komar2, Maria Vulf3, Egor Shunkin4, Larisa Litvinova5, Elena Kirienkova6
1Immanuel Kant Baltic Federal University, Dariask@list.ru
2Immanuel Kant Baltic Federal University, alexandkomar@gmail.com
3Immanuel Kant Baltic Federal University, mary-jean@yandex.ru
4Immanuel Kant Baltic Federal University, egor.shunkin@gmail.com
5Immanuel Kant Baltic Federal University, larisalitvinova@yandex.ru
6Immanuel Kant Baltic Federal University, elenamed@list.ru

According to the world community, metabolic complications are associated with inflammation in obesity. The cytokines of the interleukins (IL) – 6 family consist of IL-6, IL-11, IL-27 and have similar, but individual effects, such as the regulation of liver damage, the balance between regulatory and effector T cells and metabolic regulation. The anti-inflammatory and regenerative activities of IL-6 are usually mediated by the classical signaling regimen, while the pro-inflammatory responses in many pathological conditions include trans-signaling. The aim of the study was to assess the role of the cytokines of the IL-6 family: IL-6, sIL-6Ra, gp130 / sIL-6Rb, IL-11 in the regulation of carbohydrate (glucose level) and lipid (cholesterol (Hol), high-density lipoprotein (HDL), low-density lipoprotein (LDL), triglycerides (TR)) exchanges in serum / plasma in patients with metabolic syndrome (MetS). This fact may indicate the protective role of sIL-6Ra trans-signaling and protective role of IL-6 signal inhibition due to high levels of sgp130 / sIL-6Rb in relation to metabolic disorders in obesity patients.

Systems Biology and Biomedicine symposiumMetabolic response of the Siberian frog Rana amurensis to anoxia

Metabolic response of the Siberian frog Rana amurensis to anoxia

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Sergei V. Shekhovtsov1, Nina A. Bulakhova2, Yuri P. Tsentalovich3, Ekaterina A. Zelentsova4, Daniil I. Berman5
1ICG SB RAS, Novosibirsk, Russia, shekhovtsov@bionet.nsc.ru
2IBPN FEB RAS, Magadan, Russia, sigma44@mail.ru
3ITC SB RAS, Novosibirsk, Russia, yura.tsentalovich@tomo.nsc.ru
4ITC SB RAS, Novosibirsk, Russia, zelentsova.ekaterina@gmail.com
5IBPN FEB RAS, Magadan, Russia, dberman@mail.ru

The Siberian frog Rana amurensis is the only amphibian capable of surviving several months at almost complete anoxia. We performed 1H NMR metabolomics analysis for the liver and heart of the Siberian frog during normoxia and anoxia. We found that anoxia causes significant energetic stress with much less energy molecules available in the studied organs. This is accompanied by the accumulation of several end products, most significantly lactate, alanine, and succinate, as well as most amino acids.

Systems Biology and Biomedicine symposiumMIRNA EXPRESSION PROFILE IN ABNORMALLY INVASIVE PLACENTA: ACCRETA, INCRETA AND PERCRETA CASES

MIRNA EXPRESSION PROFILE IN ABNORMALLY INVASIVE PLACENTA: ACCRETA, INCRETA AND PERCRETA CASES

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Poster (download)

[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/403.pdf”]
Angelika Timofeeva1, Oksana Vasilchenko2, Mariya Pirogova3, Ivan Fedorov4, Vitaliy Chagovets5, Larisa Ezhova6, Roman Shmakov7, Gennadiy Sukhikh8
1FSBI В«National Medical Research Center For Obstetrics, Gynecology And Perinatology Named After Academician V.I.KulakovВ» Ministry of Healthcare of the Russian Federation, Moscow, Russia, avtimofeeva28@gmail.com
2FSBI В«National Medical Research Center For Obstetrics, Gynecology And Perinatology Named After Academician V.I.KulakovВ» Ministry of Healthcare of the Russian Federation, Moscow, Russia, vasilchenko-on@mail.ru
3FSBI В«National Medical Research Center For Obstetrics, Gynecology And Perinatology Named After Academician V.I.KulakovВ» Ministry of Healthcare of the Russian Federation, Moscow, Russia, pirogovamariya@gmail.com
4FSBI В«National Medical Research Center For Obstetrics, Gynecology And Perinatology Named After Academician V.I.KulakovВ» Ministry of Healthcare of the Russian Federation, Moscow, Russia, i_fedorov@oparina4.ru
5FSBI В«National Medical Research Center For Obstetrics, Gynecology And Perinatology Named After Academician V.I.KulakovВ» Ministry of Healthcare of the Russian Federation, Moscow, Russia, vvchagovets@gmail.com
6FSBI В«National Medical Research Center For Obstetrics, Gynecology And Perinatology Named After Academician V.I.KulakovВ» Ministry of Healthcare of the Russian Federation, Moscow, Russia, l_ezhova@oparina4.ru
7FSBI В«National Medical Research Center For Obstetrics, Gynecology And Perinatology Named After Academician V.I.KulakovВ» Ministry of Healthcare of the Russian Federation, Moscow, Russia, r_shmakov@oparina4.ru
8FSBI В«National Medical Research Center For Obstetrics, Gynecology And Perinatology Named After Academician V.I.KulakovВ» Ministry of Healthcare of the Russian Federation, Moscow, Russia, g_sukhikh@oparina4.ru

It is well-known that abnormal placental invasion is a condition characterized by pathological invasion of chorionic villi into the the basal layer of the uterus that is complicated by massive obstetric hemorrhage, blood transfusions, a need for hysterectomy and even mortality. The altered miRNA expression profiles were found by deep sequencing not only directly in the area of pathological invasion, but also in the outside distant placenta areas with more pronounced changes in the former case. An increase in the level of miR-21-5p, miR-25-3p, miR-92a-3p, miR-320a in maternal plasma may indicate abnormally invasiveВ placenta and potentially be used in the early diagnosis and management of this condition.

Systems Biology and Biomedicine symposiumThe effect of round-the-clock illumination on blood hemoglobin level, body composition and endurance of C57Bl/6 mice

The effect of round-the-clock illumination on blood hemoglobin level, body composition and endurance of C57Bl/6 mice

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Poster (download)

[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/47.pdf”]
Irina Yurievna Ishchenko1, Svetlana Viktorovna Michurina2, Andrey Yurievich Letyagin3, Maxim Aleksandrovich Korolev4, Lyubov Nikiforovna Rachkovskaya5, Anna Stanislavovna Khotskina6, Nikita Valeryevich Khotskin7, Svetlana Olegovna Maslennikova8, Evgenii L. Zavjalov9
1Group of experimental pharmacology SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, irenisch@mail.ru
2Group of experimental pharmacology SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, s.michurina@ngs.ru
3Laboratory of pharmaceutical technologies SRICEL – a branch of ICG SB RAS, letyagin-andrey@yandex.ru
4Laboratory of connective tissue pathology SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, kormax@bk.ru
5Laboratory of pharmaceutical technologies SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, noolit@niikel.ru
6Center of genetic resources of laboratory animals ICG SB RAS Novosibirsk, Russia, dotcenko@bionet.nsc.ru
7Sector of genetic collections of neuropathologies ICG SB RAS Novosibirsk, Russia, shverter@mail.ru
8Center of genetic resources of laboratory animals ICG SB RAS Novosibirsk, Russia, maslennikova@bionet.nsc.ru
9Center of genetis resources of laboratory animals ICG SB RAS Novosibirsk, Russia, zavjalov@bionet.nsc.ru

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Эффекты круглосуточного освещения были изучены на самцах мышей C57Bl / 6. Масса тела мышей увеличилась после двух недель 24-часового освещения (24 часа). Рост массы тела был отмечен за счет увеличения мышечной массы. Уровень гемоглобина в крови снизился в конце эксперимента. Мы обнаружили снижение общего времени зависания на 14-й день эксперимента.

Systems Biology and Biomedicine symposiumNonvascular pathways of aqueous humor outflow in the choroid of the human eye

Nonvascular pathways of aqueous humor outflow in the choroid of the human eye

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Poster (download)

[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/115.pdf”]
Sabina Nogovitsina1
1Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, nogovitsina.niikel@gmail.com

The structural organization of lymphatic-like channels in the human eye choroid was studied by using immunohistochemistry and transmission electron microscopy. It was shown that these channels lining fibroblast-like cells that express markers of lymphatic endothelium.