International Multiconference “Bioinformatics of Genome Regulation and Structure\Systems Biology”

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Systems Biology and Biomedicine symposium

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Systems Biology and Biomedicine symposiumEndoglycosidase expression in pubocervical fascia is up-regulated in menopause patients with severe pelvic organs prolapse

Endoglycosidase expression in pubocervical fascia is up-regulated in menopause patients with severe pelvic organs prolapse

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Poster (download)

[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/116.pdf”]
Svetlana V. Aidagulova1, Fedor A. Rakitin2, Mikhail Yu. Soluyanov3, Vadim V. Nimaev4, Igor O. Marinkin5
1Novosibirsk State Medical University, s.aydagulova@gmail.com
2Research Institute of Clinical and Experimental Lymphology – Branch ICG SB RAS, rakitinfedorr@mail.ru
3Research Institute of Clinical and Experimental Lymphology – Branch ICG SB RAS, msoluyanov@mail.ru
4Research Institute of Clinical and Experimental Lymphology – Branch ICG SB RAS, nimaev@gmail.com
5Novosibirsk State Medical University, rectorngmu@yandex.ru

Pelvic organs prolapse in women is very inconvenient age-dependent pathology. One of the causes of pelvic organs prolapse is considered so called undifferentiated dysplasia of connective tissue being genetically determined change in the morphogenesis of connective tissue. The aim of this work was to study heparanase expression in pubocervical fascia of menopause women with pelvic organ prolapse. As shown heparanase protein content was significantly up-regulated in cellular compartment of pubocervical fascia in patients with pelvic organs prolapse and prominent undifferentiated dysplasia of connective tissue degrees.В 

Systems Biology and Biomedicine symposiumThe structure of the cytokine gene network in uterine fibroids

The structure of the cytokine gene network in uterine fibroids

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Poster (download)

[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/46.pdf”]
Viktor Prokofiev1, Alla Shevchenko2, Vladimir Konenkov3, Nikolay Orlov4, Elena Koroleva5, Alexey Novikov6
1Research Institute of Clinical and Experimental LymСЂhology – Branch of the Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, vf_prok@mail.ru
2Research Institute of Clinical and Experimental LymСЂhology – Branch of the Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, shalla64@mail.ru
3Research Institute of Clinical and Experimental LymСЂhology – Branch of the Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, vikonenkov@gmail.com
4Research Institute of Clinical and Experimental LymСЂhology – Branch of the Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, nbo700@mail.ru
5Research Institute of Clinical and Experimental LymСЂhology – Branch of the Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, lymphology@niikel.ru
6Research Institute of Clinical and Experimental LymСЂhology – Branch of the Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, novis.ngmu@gmail.com

The structure of 166416 gene chains of various dimensions consisting of multilocus combinations of genes of 12 polymorphic sites forming a cytokine gene network associated with the development of uterine fibroids (UF) was studied.В  Using the Cytoscape bioinformation platform, computer modeling of network interactions of cytokine genotypes of various nature involved in the regulation of inflammation, angiogenesis, destruction and remodeling of connective tissue involved in the pathogenesis of polygenic diseases, including UF, was carried out.В  An analysis of the topology of this gene network made it possible to identify the main genes and the main intergenic interactions that make the greatest contribution to the development of UF.В  In our opinion, 3 polymorphisms can act as the main genes: IL1B-31TC, VEGF + 936CT and IL10-592CA.В  These genes form the main nodes in the gene network because they have the largest number of interactions with other genes, which account for 55% of all pair interactions in the gene network, which forms a predisposition to the development of UF.

Systems Biology and Biomedicine symposiumThe tendency to hypercoagulation in the perioperative period of uterine artery embolization.

The tendency to hypercoagulation in the perioperative period of uterine artery embolization.

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Poster (download)

[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/33.pdf”]
Irina Siutkina1, Khabarov Dmitriy2, Smagin Alexandr3, Bulichev Pavel4, Demura Alexander5, Inyoshina Alisa6
1Research Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, komarok777@mail.ru
2Research Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, hdv@ngs.ru
3Research Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, asa57@ngs.ru
4Research Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, paulbulychev@gmail.com
5Research Institute of Clinical and Experimental Lymрhology – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, dx_@bk.ru
6Novosibirsk State University, V.Zelman Institute for Medicine and Psychology 1, Pirogova str., Novosibirsk , Russia, alisaineshinansk@gmail.com

To assess the dynamics of changes in the blood coagulation system in the perioperative period during uterine artery embolization (EUA) based on monitoring of hemostasis system’s indicators, including TEG, and the tendency (or its absence) to trombosis.

Systems Biology and Biomedicine symposiumInflammation is associated with desynchronosis in the immune system (experimental study).

Inflammation is associated with desynchronosis in the immune system (experimental study).

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Poster (download)

[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/31.pdf”]
Abdalova A.M.1, Shurlygina A.V.2, Dergacheva T.I.3, Klimontov V.V.4, Letyagin A.Yu.5
1Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics SB RAS, arzuabd@mail.ru
2Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics SB RAS, anna_v_s@mail.ru
3Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics SB RAS, dr-tanja@yandex.ru
4Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics SB RAS, klimontov@mail.ru
5Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics SB RAS, letyagin-andrey@yandex.ru

The circadian temporary organization of the dynamic processes occurring at the level of the cellular compartment of the immune system ensures their clear time sequence, the optimal ratio of cellular elements in organs and tissues at any given time/ Any pathological process leads to biorhythmological disorders of varying severity, which begin at the very early stages of the disease, when clinical signs are not yet manifested. However, in the literature there is very little information about the daily biorhythms of the immune system in violation of its functions, in particular, in the chronic inflammatory process. We found that with inflammation in the thymus, spleen and lymph nodes, the nature of the diurnal fluctuations in the content of large, medium and small lymphocytes, monocytes / macrophages, CD8 +, CD25 + cells changes. Thus, with the development of inflammation in the uterine mucosa in rats, desynchronosis in the immune system is observed. This allows us to talk about the need to take into account circadian rhythms in the diagnosis and treatment of inflammatory diseases.

 

Systems Biology and Biomedicine symposiumMolecular diagnostics of hearing loss due to mutations in the SLC26A4 gene in indigenous peoples of Southern Siberia (Russia)

Molecular diagnostics of hearing loss due to mutations in the SLC26A4 gene in indigenous peoples of Southern Siberia (Russia)

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Poster (download)

[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/29.pdf”]
Valeriia Yuryevna Danilchenko1, Marina Vyacheslavovna Zytsar2, Marita Sergeevna Bady-Khoo3, Ekaterina Alexandrovna Maslova4, Olga Leonidovna Posukh5
1Federal Research Center Institute of Cytology and Genetics, danilchenko_valeri@mail.ru
2Federal Research Center Institute of Cytology and Genetics, zytzar@bionet.nsc.ru
3Perinatal Center of the Republic of Tyva, marita.badyhoo@mail.ru
4Federal Research Center Institute of Cytology and Genetics; Novosibirsk State University, maslova@bionet.nsc.ru
5Federal Research Center Institute of Cytology and Genetics; Novosibirsk State University, posukh@bionet.nsc.ru

Establishing a genetic diagnosis of hearing loss is of great importance for clinical evaluation of deaf patients and for estimating recurrence risks for their families. Mutations in the SLC26A4 gene are a common cause of hearing loss in many regions of the world. This study presents the results of the first molecular genetic analysis of the SLC26A4 gene sequence performed in patients with hearing loss of unknown etiology belonging to indigenous peoples of Southern Siberia – Tuvinians (the Tyva Republic) and Altaians (the Altai Republic). Contrast differences of the SLC26A4 pathogenic contribution to the etiology of hearing impairment were revealed: 28.2% for Tuvinians and 4.3% for Altaians. Both known and novel pathogenic variants as well as a wide range of polymorphic variants were found in the SLC26A4 gene sequences of examined patients. High frequency of mutation c.919-2A>G in Tuvinians is probably due to the founder effect.

Systems Biology and Biomedicine symposiumLoci and genes involved in chronic musculoskeletal pain identified via analysis of genetically independent pain phenotypes

Loci and genes involved in chronic musculoskeletal pain identified via analysis of genetically independent pain phenotypes

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Yakov Tsepilov1, Maxim B. Freidin2, Alexandra S. Shadrina3, Sodbo Z. Sharapov4, Elizaveta E. Elgaeva5, Jan van Zundert6, Lennart РЎ. Karssen7, Pradeep Suri8, Frances M.K. Williams9, Yurii S. Aulchenko10
1Novosibirsk State University, drosophila.simulans@gmail.com
2King’s College London, maxim.freydin@kcl.ac.uk
3Novosibirsk State University, weiner.alexserg@gmail.com
4Novosibirsk State University, sharapovsodbo@gmail.com
5Novosibirsk State University, elizabeth.elgaeva@gmail.com
6Maastricht University Medical Centre, jan.vanzundert@zol.be
7PolyOmica, l.c.karssen@polyomica.com
8VA Puget Sound Health Care System, pradeepsuri1@gmail.com
9King’s College London, frances.williams@kcl.ac.uk
10Novosibirsk State University, y.s.aulchenko@polyomica.com

We have evaluated four genetically independent pain phenotypes of four common chronic musculoskeletal pains (GIPs). We assume that the first GIP represents a biopsychological component of chronic musculoskeletal pain, related to physiological and psychological aspects and possibly reflecting pain perception and processing.

Systems Biology and Biomedicine symposiumProteins and Cytokines of Blood Cells Supernatant and Histological Atypical Cells Markers in the Breast Diseases

Proteins and Cytokines of Blood Cells Supernatant and Histological Atypical Cells Markers in the Breast Diseases

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Poster (download)

[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/26.pdf”]
Video (download)

Kristina Davletova1, Albina Bernado2, Sergey Arkhipov3, Igor Zhurakovsky4, Nikolay Varaksin5, Alexander Autenshlyus16
1Novosibirsk State Medical University, Institute of Molecular Biology and Biophysics – subdivision of FRC FTM, christina.davletova@gmail.com
2Novosibirsk State Medical University, idontbelive69@mail.ru
3Novosibirsk State Medical University, Institute of Molecular Biology and Biophysics – subdivision of FRC FTM, arhipowsergei@yandex.ru
4Novosibirsk State Medical University, Institute of Molecular Biology and Biophysics – subdivision of FRC FTM, murash2003@yandex.ru
5AO Vector-Best, varaksin@vector-best.ru
6Novosibirsk State Medical University, Institute of Molecular Biology and Biophysics – subdivision of FRC FTM, lpciip@211.ru

The study examined the concentration of proteins and cytokines in the supernatants of blood cells, as well as the expression of histological markers of atypical cells in breast diseases. In addition, he studies the correlation between concentrations of proteins and cytokines and histological markers of atypical cells. It was found that the concentration of histidine-rich glycoprotein in supernatants of blood cells with a special type of invasive carcinoma is higher compared with benign breast diseases. In addition, the following statistical relationships were observed in the study: negative correlation between estrogen receptor-α concentrations and integrin-1 expression; a positive correlation between the concentrations of histidine-rich glycoprotein and expression of type II collagen; a positive correlation between e-cadherin concentrations and e-cadherin expression; and a positive correlation between the concentrations of plasminogen-1 activator inhibitor and the expression of type II collagen. The production of cytokines by blood cells is identified as an index of the influence of polyclonal activators (a combination of mitogens). The study shows the difference in the index of influence on the production of IL-1RA and TNF-α between benign breast diseases with a high and low risk of malignant transformation and between benign breast diseases with a high risk of malignant transformation and breast cancer with metastases to the lymph nodes. The index of the effect of polyclonal activators on IL-1RA production increases as the prognosis of the disease worsens. As blood samples and tumors show, patients with benign breast diseases have a negative correlation between the index of influence of polyclonal activators on IL-1RA production and expression of type II collagen. In addition, patients with breast cancer as well as breast cancer with metastases in the lymph nodes have a positive correlation between the expression of e-cadherin and spontaneous and polyclonal stimulation stimulated by the production of IL-1RA. The results of the study showed that the concentrations of histidine-rich glycoprotein and IL-1RA in blood cell supernatants and the expression of e-cadherin can be used as markers for the early diagnosis of benign breast diseases with a risk of malignant transformation. and breast cancer with metastases to the lymph nodes have a positive correlation between the expression of e-cadherin and spontaneous and polyclonal activator-stimulated production of IL-1RA. The results of the study showed that the concentrations of histidine-rich glycoprotein and IL-1RA in blood cell supernatants and the expression of e-cadherin can be used as markers for the early diagnosis of benign breast diseases with a risk of malignant transformation. and breast cancer with metastases to the lymph nodes have a positive correlation between the expression of e-cadherin and spontaneous and polyclonal activator-stimulated production of IL-1RA. The results of the study showed that the concentrations of histidine-rich glycoprotein and IL-1RA in blood cell supernatants and the expression of e-cadherin can be used as markers for the early diagnosis of benign breast diseases with a risk of malignant transformation.

Systems Biology and Biomedicine symposiumMICRORNAS (221, 429) ARE CORRELATION WITH LYMPHOCYTES OF AXILLARY LYMPH NODES IN EXPERIMENTAL BREAST CANCER

MICRORNAS (221, 429) ARE CORRELATION WITH LYMPHOCYTES OF AXILLARY LYMPH NODES IN EXPERIMENTAL BREAST CANCER

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Poster (download)

[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/17.pdf”]
Aleksey Kabakov1, Alexandr Lykov2, Oleg Kazakov3, Alexandr Poveshchenko4, Olga Poveshchenko5, Dmitriy Strunkin6, Lyudmila Gulyaeva7, Andrey Letyagin8, Vladimir Konenkov9
1RICEL-Branch of IC&G SB RAS, kabakov_av@mail.ru
2RICEL-Branch of IC&G SB RAS, aplykov2@mail.ru
3RICEL-Branch of IC&G SB RAS, kazakoff_oleg@mail.ru
4RICEL-Branch of IC&G SB RAS, poveshchenkoa200@mail.ru
5RICEL-Branch of IC&G SB RAS, poveschenkoov@yandex.ru
6RIMBB FRC FTM, strunkind@mail.ru
7RIMBB FRC FTM, gulyaeva@soramn.ru
8RICEL-Branch of IC&G SB RAS, letyaginay@bionet.nsc.ru
9RICEL-Branch of IC&G SB RAS, vikonenkov@gmail.com

The study of concentration miRNA (21, 221, 222, and 429) in serum, lymph of the thoracic duct and breast cancer tissue, as well as the cellular composition of axillary lymph node in Wistar female rats in chemically induced breast cancer. Concentration microRNA (-221, -429) in the tissues breast cancer and lymph are correlated with a decrease in number of lymphocytes in medullary cords of axillary lymph nodes.

Systems Biology and Biomedicine symposiumCYTOKINES – MARKERS OF ONCOGENESIS AND THERAPY EFFICIENCY IN CHEMICALLY INDUCED BREAST CANCER

CYTOKINES – MARKERS OF ONCOGENESIS AND THERAPY EFFICIENCY IN CHEMICALLY INDUCED BREAST CANCER

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Poster (download)

[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/16.pdf”]
Alexandr Poveshchenko1
1Research Institute of Clinical andExperimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences Novosibirsk, Russia, poveshchenkoa200@mail.ru

Breast cancer was induced by the administration of n-methyl-N-nitrosourea to Wistar rats. Some animals underwent only surgical intervention or only polychemotherapy (cyclophosphamide, methotrexate, 5-ftorouracil). In some animals, both types of therapy were combined, and in a separate group, the administration of the Panagen drug, which was a fragmented DNA molecule, was added to polychemotherapy. To study the concentration of cytokines in lymph, we used the Bio-Plex Pro Rat Cytokoness 24-Plex Assay test system (Bio-Rad, USA). It was shown that in rats with breast cancer the content in the lymph and blood of most of the studied cytokines such as IL-1ОІ, IL-2, IL-4, IL-6, IL-7, IL-12, IL-13, MIP-1О±, MIP-3О±, RANTES, TNF-О±, MCP-1 were statistically significantly higher, and IL-10 and GRO / KC, lower than in intact animals. Surgical removal of the tumor led to a significant decrease in the content of both pro-inflammatory and anti-inflammatory cytokines in the lymph. PCT led to a significant decrease in the content of IL-1ОІ, IL-4, IL-6, IL-7, MIP-1О±, MIP-3О±, RANTES in the blood serum and lymph of rats with breast cancer.

Systems Biology and Biomedicine symposiumRelationship Cytokines Lymphs and Mesenteric Lymph Nodes Rats at Chemotherapy Breast Cancer

Relationship Cytokines Lymphs and Mesenteric Lymph Nodes Rats at Chemotherapy Breast Cancer

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Poster (download)

[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/15.pdf”]
Kazakov Oleg1, Poveshchenko Alexander2, Orlov Nikolai3, Kabakov Alexey4, Lykov Alexander5, Strunkin Dmitry6, Konenkov Vladimir7
1Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences, kazakoff_oleg@mail.ru
2Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences, poveshchenkoa200@mail.ru
3Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences, nbo@ngs.ru
4Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences, kabakov_av85@mail.ru
5Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences, aplykov2@mail.ru
6Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences, Strunkind@mail.ru
7Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics,Siberian Branch of the Russian Academy of Sciences, vikonenkov@gmail.com

At correlating the morphometry of mesenteric lymph nodes and the concentration of cytokines in the lymph of the thoracic duct in breast cancer induced by intramammary administration of N-methyl-n-nitrosourea, chemotherapy according to the CMF scheme, compared with breast cancer without treatment, positive relationships were found that may indicate on increase in the immunomodulatory and antitumor action of cytokines. The correlation of interferon IFNОі with small lymphocytes (the number which increased) and macrophages in germinative centers and mitotic dividing cells in medullary substance, correlation in germinative centers of immunoblasts with MIP-1О± and increase of number small lymphocytes in the thymus dependent zone of lymph nodes, correlation in medullary substance of interleukin IL-17 with mature plasma cells (the number of which is increased), correlation of interleukin IL-18 with mature plasma cells in the medullary sinuses.