International Multiconference “Bioinformatics of Genome Regulation and Structure\Systems Biology”

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Systems Biology and Biomedicine symposium

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Systems Biology and Biomedicine symposiumDysbiosis in the Gut Microbiota of Adolescents with Obesity

Dysbiosis in the Gut Microbiota of Adolescents with Obesity

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Novikova Evgenia Anatolyevna1, Bairova Tatyana Ananyevna2, Belkova Natalia Leonidovna3, Pogodina Anna Valeryevna4, Romanitsa Anastasia Igorevna5, Rychkova Lyubov Vladimirovna6
1Scientific РЎentre of Family Health and Human Reproduction Problems, europe411@mail.ru
2Scientific РЎentre of Family Health and Human Reproduction Problems, tbairova38@mail.ru
3Scientific РЎentre of Family Health and Human Reproduction Problems, nlbelkova@gmail.com
4Scientific РЎentre of Family Health and Human Reproduction Problems, pogodina_av@inbox.ru
5Scientific РЎentre of Family Health and Human Reproduction Problems, f1693@rambler.ru
6Scientific РЎentre of Family Health and Human Reproduction Problems, rychkova.nc@gmail.com

Gut microbiota plays a fundamental role in the pathogenesis of metabolic disorders including obesity. Gut microbial dysbiosis induces immune and metabolic disturbances. We wanted to find out a gut microbiota composition at adolescents with obesity and normal weight. The examined group included 40 adolescents. There were 18 obese adolescents with SDS BMI=2,77В±0,55 in the main group, and 22 adolescents with normal weight (SDS BMI=0,01В±0,50) in the control group. The metagenome sequencing of V3-V4 variable regions of 16S rDNA was done by Novogene Company (China).В  Data were analyzed using the StatSoft STATISTICA 10.0 software package. Statistical significance was accepted at the p<0.05 level. We did not find any variations between two groups in the main phylum Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, and following genera Bacteroides, Alistipes, Subdoligranulum, Megasphaera, Blautia, Akkermansia, Odoribacter, Lactobacillus, Bifidobacterium. But for phylotypes Enterobacter and Anaerotruncus the differences in gut microbiota composition were found. The obese participants had a 2-fold decrease in Enterobacter (p=0.02), and an increase – in the Anaerotruncus phylotype (p=0.04). Summing up the results we might say that adolescents with obesity had decreased the number of the phylum Proteobacteria (the Enterobacter phylotype) and increased the level of the phylum Firmicutes (the Anaerotruncus phylotype).

Systems Biology and Biomedicine symposiumThe expression of Bcl-2 family proteins in liver cells of РЎ57Bl/6 mice under conditions of functional pinealectomy

The expression of Bcl-2 family proteins in liver cells of РЎ57Bl/6 mice under conditions of functional pinealectomy

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Poster (download)

[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/188.pdf”]
Svetlana Viktorovna Michurina1, Irina Yurievna Ishchenko2, Sergey Alekseevich Arkhipov3, Andrey Yurievich Letyagin4, Maxim Aleksandrovich Korolev5, Evgenii Leonidovich Zavjalov6
1Group of experimental pharmacology SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, s.michurina@ngs.ru
2Group of experimental pharmacology SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, irenisch@mail.ru
3Group of experimental pharmacology SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, arhipowsergei@yandex.ru
4Laboratory of pharmaceutical technologies SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, letyagin-andrey@yandex.ru
5Laboratory of connective tissue pathology SRICEL – a branch of ICG SB RAS Novosibirsk, Russia, kormax@bk.ru
6Center of genetis resources of laboratory animals ICG SB RAS Novosibirsk, Russia, zavjalov@bionet.nsc.ru

The expression of antiapoptotic Bcl-2 protein and the proapoptotic Bad protein in liver cells of C57BL/6 mice males kept 14 days under 24-hour lighting (24hL) (photoperiod light/dark 24/0 hours) was studied. Control animals kept under standard lighting conditions (photoperiod light/dark 14/10 hours). The immunohistochemical analysis (indirect avidin-biotin-streptavidin method) and morphometric assessment were used. We found in the liver cells of mice under round-the-clock lighting the increase in the area of Bcl-2 protein expression without changing the area of Bad protein expression, that led to a decrease in the ratio of Bcl-2/Bad. This result indicated weakening of the antiapoptotic protection of organ cells and creates conditions for activation of the “mitochondrial branch” of apoptosis in animal liver cells with functional pinealectomy.

Systems Biology and Biomedicine symposiumSyndecan-1 expression in ovarian endometrioid cancer tissue has negative correlation with estrogen status

Syndecan-1 expression in ovarian endometrioid cancer tissue has negative correlation with estrogen status

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Alexander V. Volchek1, Yulia S. Timofeeva2, Yanina M. Evseeva3, Dmitriy V. Morozov4, Igor O. Marinkin5, Svetlana V. Aidagulova6
1Novosibirsk State Medical University, alexander@volcheck.ru
2Novosibirsk State Medical University, dr.yustimofeeva@gmail.com
3Novosibirsk State Medical University, janinaevseeva@yahoo.com
4City Clinical Hospital в„–1, mdvil07@mail.ru
5Novosibirsk State Medical University, rectorngmu@yandex.ru
6Novosibirsk State Medical University, s.aydagulova@gmail.com

Cancer treatment shifts from mono-targeted to combination therapy, considering the tumor microenvironment with heparan sulfates, including Syndecan-1 (SDC1). The aim of this work was to study correlation between core-protein SDC1 and estrogen receptors expression in surgical material of 15 women aged 53,0В В±В 10,9 years with endometrioid ovarian cancer (EOC) using immunohistochemistry and automatic image analysis. The staining intensity of SDC1 in tumor tissue was 1304,23В В±В 65,85 units, the staining intensity of the estrogen receptors as 1137,88В В±В 135,40 units. The significant inverse correlation (RВ =В -В 0,512, p = 0,003) between SDC1 and estrogen receptors expression levels were obtained. Most likely, this deals with progressive character of EOC.

Systems Biology and Biomedicine symposiumHuman retinal photoreceptor cells in glaucoma: destructive changes of mithochondria and mitophagy

Human retinal photoreceptor cells in glaucoma: destructive changes of mithochondria and mitophagy

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[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/175.pdf”]
Natalia Obanina1, Nataliya Bgatova2, Valerii Chernykh3, Aleksandr Trunov4, Alena Eremina5, Vladimir Konenkov6
1Laboratory of ultrastructural research, Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences; Section of cytology and genetics, Department of Natural Sciences, Novosibirsk State University, n.obanina@g.nsu.ru
2Laboratory of ultrastructural research, Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, n_bgatova@ngs.ru
3The academician S.N. Fyodorov Federal State Institution “Intersectoral Research and Technology Complex “Eye Microsurgery”, Ministry of Healthcare of Russian Federation, Novosibirsk Branch, rimma@mntk.nsk.ru
4The academician S.N. Fyodorov Federal State Institution “Intersectoral Research and Technology Complex “Eye Microsurgery”, Ministry of Healthcare of Russian Federation, Novosibirsk Branch, trunov1963@yandex.ru
5The academician S.N. Fyodorov Federal State Institution “Intersectoral Research and Technology Complex “Eye Microsurgery”, Ministry of Healthcare of Russian Federation, sci@mntk.nsk.ru
6Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, vikonenkov@gmail.com

The leading place in the development of low vision and blindness worldwide is occupied by glaucoma. This disease is non-infectious in nature, accompanied by an increase in intraocular pressure and degeneration of neurons of the retina, optic nerve, as well as its spread in the central part of the visual analyzer. The aim of the study was to identify the characteristics of the ultrastructure of human retinal photoreceptor cells in glaucoma. The study was carried out using transmission electron microscopy and morphometry. The volume densities (VV) of mitochondria and autophagosomes containing mitochondria were determined. Disorders of orientation and swelling of the membrane discs of rod neurons in the outer segments were revealed. It is shown that sticks are more prone to destructive changes than cones.

Systems Biology and Biomedicine symposiumHuman Dermal Fibroblasts and Bone-Marrow Mesenchymal Stem Cells properties under Silver and Lithium Condition

Human Dermal Fibroblasts and Bone-Marrow Mesenchymal Stem Cells properties under Silver and Lithium Condition

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[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/12.pdf”]
Alexander Lykov1, Lubov Rachkovskaya2, Olga Poveshchenko3, Maria Surovtseva4, Irina Kim5, Edmund Rachkovsky6, Alena Philippova7
1Research Institute of Clinical ans Experimental Lymphology- Branch of the Institute of Cytology and Genetics SB RAS, aplykov2@mail.ru
2Research Institute of Clinical ans Experimental Lymphology- Branch of the Institute of Cytology and Genetics SB RAS, lymphology@niikel.ru
3Research Institute of Clinical ans Experimental Lymphology- Branch of the Institute of Cytology and Genetics SB RAS, poveschenkoov@yandex.ru
4Research Institute of Clinical ans Experimental Lymphology- Branch of the Institute of Cytology and Genetics SB RAS, mfelde@ngs.ru
5Research Institute of Clinical ans Experimental Lymphology- Branch of the Institute of Cytology and Genetics SB RAS, kii5@yandex.ru
6Research Institute of Clinical ans Experimental Lymphology- Branch of the Institute of Cytology and Genetics SB RAS, lymphology@niikel.ru
7Municipal autonomous educational institution Education center Gornostay, alena.philippova2003@yandex.ru

Fibroblasts and mesenchymal stem cells are involved in damaged skin repair. Functional activity of the fibroblasts mesenchymal stem cells depends from environment, including antimicrobial preparations. Silver salts are one of the antimicrobial substances. Lithium salts exhibit not only a stabilizing effect on human mental responses, but also antitumor and autophagic effects. The “Bodyguard” hygienic composition contains both silver and lithium. The aim of the study was to study the effect of bound silver and lithium ions in the \”Bodyguard\” composition and in salt of silver nitrate and lithium of citric acid on human dermal fibroblasts (DFs) and bone-marrow mesenchymal stem cells (MSCs) functions. It has been shown that DFs in the presence of \”Bodyguard\” reduces proliferative activity and significantly increases care in apoptosis. At the same time, silver and lithium ions alone or in combination do not affect proliferation, enhance migration and reduce DFs apoptosis. It has been shown that MSCs proliferation increased under “Bodyguard” condition. Whereas, silver ions and lithium ions not have any effect on proliferation of MSCs, but influenced on myeloperoxidase activity, nitric oxide production, migration, and apoptosis. The more pronounced negative action of the “Bodyguard” is most likely due not to silver and lithium ions, but to the very structure of the carrier to which these ions are immobilized.

Systems Biology and Biomedicine symposiumMarkers of oxidative and atherogenic processes in individuals with hand-arm vibration syndrome and metabolic disorders

Markers of oxidative and atherogenic processes in individuals with hand-arm vibration syndrome and metabolic disorders

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Video (download)

Masnavieva Liudmila1, Kudaeva Irina2, Chistova Nadezhda3
1East-Siberian Institute of Medical and Ecological Research, Masnavieva_luda@mail.ru
2East-Siberian Institute of Medical and Ecological Research, Kudaeva_Irina@mail.ru
3East-Siberian Institute of Medical and Ecological Research, chist1nad2pavl3@gmail.com

Changes in the activity of lipid peroxidation processes are observed when exposed to vibration, as well as metabolic syndrome and type 2 diabetes, which are characterized by metabolic disorders. This study is devoted to the assessment of atherogenic and oxidative processes in individuals with hand-arm vibration syndrome in combination with metabolic disorders. We examined the levels of cholesterol, cholesterol in high and low density lipoproteins, oxidized low density lipoproteins, antibodies to them and the thiol status in 59 men with hand-arm vibration syndrome, in 73 people with vibrational disease in combination with metabolic syndrome and in 35 individuals with hand-arm vibration syndrome and type 2 diabetes. It was found that the average value of total cholesterol was higher than the reference level in all groups. A decrease in cholesterol in high density lipoproteins and an increase in cholesterol in low density lipoproteins have been observed in individuals with metabolic disorders. Levels of oxidized low-density lipoproteins and antibodies to them were increased in all groups. Correlation existed between the level of total cholesterol and cholesterol in low density lipoprotein, between the content of oxidized low density lipoproteins and total cholesterol, between the concentration of oxidized low density lipoproteins and cholesterol in low density lipoprotein in all groups. The correlation between the cholesterol level in high lipoproteins and thiol status was disrupted in patients with hand-arm vibration syndrome and metabolic disorders.

Systems Biology and Biomedicine symposiumFunctional study of potential regulatory SNPs

Functional study of potential regulatory SNPs

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Arina Degtyareva1, Elena Leberfarb2, Ilya Brusentsov3, Tatiana Kuzina4, Tatiana Merkulova5
1ICG SB RAS, degtyareva_rso@mail.ru
2ICG SB RAS, lieberfarb@bionet.nsc.ru
3ICG SB RAS, brusentsovi@gmail.com
4NSU, t.kuzina@g.nsu.ru
5ICG SB RAS, merkulova@bionet.nsc.ru

In a previous study using a new bioinformatic approach based on Chip-Seq data with antibodies to various transcription factors , histone markers , and ChiA-PET and RNA-seq data, 32 potentially regulatory single -nucleotide polymorphisms (SNPs) were associated with colorectal cancer . In this study, a functional analysis of 6 of these polymorphisms was performed using EMSA and the luciferase reporter system. These nucleotide substitutions have been shown to alter the binding of oligonucleotides to nuclear extract proteins and alter the expression of the reporter gene.

Systems Biology and Biomedicine symposiumX-chromosome Inactivation in American Mink iPSCs

X-chromosome Inactivation in American Mink iPSCs

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[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/165.pdf”]
Inna Pristyazhnyuk1, Aleksei Menzorov2
1Institute of Cytology and Genetics SB RAS, iprist@mail.ru
2Institute of Cytology and Genetics SB RAS, menzorov@bionet.nsc.ru

Induced pluripotent stem cells (iPSCs) were produced from many species. One of the key iPSC characteristics is X-chromosome inactivation status. Both active X-chromosomes mark naГЇve pluripotency, the presence of inactive X-chromosome suggests primed pluripotency. We had previously shown that American mink embryonic stem cells have both X-chromosomes active. In this study, we produced American mink iPSCs from XX embryonic fibroblasts and studied X-chromosome inactivation. We performed immunocytochemical staining of H3K27me3 chromatin modification (marker of inactive chromatin) and reverse transcription PCR of the Xist expression (marker of inactivated X-chromosome) in single cells. We have shown that according to the immunocytochemical analysis of three iPSC lines, from 56.8 to 95.6 % of cells had both X-chromosome homologs active. Xist expression data confirmed these results for one iPSC line, but unexpectedly the majority of cells in the other two iPSC lines were positive for Xist expression, so one of the X-chromosomes was presumably inactivated. Thus, we analyzed X-chromosome inactivation in XX American mink iPSCs for the first time and revealed the presence of different X-chromosome inactivation patterns.

Systems Biology and Biomedicine symposiumEffect of bispidine containing monoterpenoid moieties on physical performance in mice

Effect of bispidine containing monoterpenoid moieties on physical performance in mice

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[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/161.pdf”]
Anastasiya A. Kotlyarova1, Konstantin Yu. Ponomarev2, Ekaterina A. Morozova3, Dina V. Korchagina4, Evgeniy V. Suslov5, Alla V. Pavlova6, Tatyana G. Tolstikova7, Konstantin P. Volcho8, Nariman F. Salakhutdinov9
1Research Institute of Clinical and Experimental Lymphology – a branch of the Institute of Cytology and Genetics of Siberian Branch of Russian Academy of Sciences, kotlyarova.anastasiya@yandex.ru
2Novosibirsk Institute of Organic Chemistry of Siberian Branch of Russian Academy of Sciences, ponomarev@nioch.nsc.ru
3Novosibirsk Institute of Organic Chemistry of Siberian Branch of Russian Academy of Sciences, ekaterina.morozoff@gmail.com
4Novosibirsk Institute of Organic Chemistry of Siberian Branch of Russian Academy of Sciences, korchaga@nioch.nsc.ru
5Novosibirsk Institute of Organic Chemistry of Siberian Branch of Russian Academy of Sciences, suslov@nioch.nsc.ru
6Novosibirsk Institute of Organic Chemistry of Siberian Branch of Russian Academy of Sciences, alla.sudos@gmail.com
7Novosibirsk Institute of Organic Chemistry of Siberian Branch of Russian Academy of Sciences, tg_tolstikova@mail.ru
8Novosibirsk Institute of Organic Chemistry of Siberian Branch of Russian Academy of Sciences, volcho@nioch.nsc.ru
9Novosibirsk Institute of Organic Chemistry of Siberian Branch of Russian Academy of Sciences, anvar@nioch.nsc.ru

Currently, increasing physical performance is an urgent task, not only in the field of extreme conditions, for the purposes of sports and military medicine, but also in the pharmacology of a healthy person. The aim of this work is to study the effect of monoterpenoid derivatives with 3,7-diazabicyclo [3.3.1] nonane (bispidine) on the physical performance of mice. Physical endurance was studied in two tests: Porsolt forced swimming test with a load of 7% of body weight and running on a treadmill. To assess the effect of the investigated agents on locomotor activity, an open field test was performed. As a result, it was found that of the two compounds with the codes K1-456 and K1-458, the most effective increase in running and swimming duration is compound K1-458, containing residues of (-) – myrtenal linked to the bispidine moiety through the amino group, at a dose of 100 mg/kg after a single intragastric administration. It was also found that the studied agents statistically significantly increase locomotor activity, distance traveled, speed and reduce the time of the stationary moment when testing in the \”open field\”. Thus, derivatives of bispidine containing monoterpenoid residues are a promising new class of organic compounds for the search for new drugs to increase health.

Systems Biology and Biomedicine symposiumCytotoxicity of Titanium Oxynitride Obtained By Reactive Magnetron Sputtered On Endothelial Cells Line EaHy 926

Cytotoxicity of Titanium Oxynitride Obtained By Reactive Magnetron Sputtered On Endothelial Cells Line EaHy 926

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[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/158.pdf”]
Maria Surovtseva1, Olga Poveshchenko2, Alexander Lykov3, Irina Kim4, Vladimir Pichugin5, Irina Zhuravleva6
1Research Institute of Clinical ans Experimental Lymphology- Branch of the Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, mfelde@ngs.ru
2E. Meshalkin National Medical Research Center of the RF Ministry of Health Novosibirsk, Russia, poveschenkoov@yandex.ru
3Research Institute of Clinical ans Experimental Lymphology- Branch of the Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, aplykov2@mail.ru
4Research Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, kii5@yandex.ru
5Tomsk Polytechnic University Tomsk, Russia, pichuginvf@mail.ru
6E. Meshalkin National Medical Research Center of the RF Ministry of Health Novosibirsk, Russia, juravl_irina@mail.ru

AbstractOne of the current areas of medical materials science is the formation of ultrathin coatings on the surface of implants. Among the many types of coatings used in vascular surgery, binary and ternary titanium compounds have good prospects due to their high bio- and hemocompatibility. The properties of the coating based on films of nitrogen-containing titanium oxide (titanium oxynitride), applied, in particular, by reactive magnetron sputtering (RMS), was studied. While, problems of establishing and maintaining the necessary spraying mode is very relevant for the RMS technique. The aim of the study was determine the cytotoxicity of titanium oxy nitride compounds obtained under various modes of functioning of RMS on endothelial cells. It was showed that the levels of nitric oxide (NO) production by EaHy 926 cell line on Day 1 and Day 5 growth under the different samples of nitinol treated with different RMS modes was comparable to control. It was no obtained cytotoxic effect of the nitrogen-containing coatings of titanium oxide obtained by various modes of RMS on EaHy 926 cell line. While, was observed reduced levels of NO production by EaHy 926 cell line on Day 3.