International Multiconference “Bioinformatics of Genome Regulation and Structure\Systems Biology”

Tag

barley

2 RESULTS
Bioinformatics and systems biology of plants symposiumIdentification of an AP2/ERF Transcription Factor Controlling the Synthesis of Barley Epicuticular Wax

Identification of an AP2/ERF Transcription Factor Controlling the Synthesis of Barley Epicuticular Wax

No Comment on Identification of an AP2/ERF Transcription Factor Controlling the Synthesis of Barley Epicuticular Wax

Poster (download)

[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/246.pdf”]
Ekaterina Kolosovskaya1, Sophia Gerasimova2, Anna Korotkova3, Christian Hertig4, Sergey Morozov5, Elena Chernyak6, Dmitriy Domrachev7, Vikhorev Alexander8, Nikolay Shmakov9, Alexey Kochetov10, Jochen Kumlehn11, Elena Khlestkina12
1ICG SB RAS, Novosibirsk, Russia, kolosovskaya@bionet.nsc.ru
2ICG SB RAS, Novosibirsk, Russia NSU, Novosibirsk, Russia, gerson@bionet.nsc.ru
3ICG SB RAS, Novosibirsk, Russia, korotkova@bionet.nsc.ru
4Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Germany, hertig@ipk-gatersleben.de
5Novosibirsk Institute of Organic Chemistry, SB RAS Novosibirsk, Russia, moroz@nioch.nsc.ru
6Novosibirsk Institute of Organic Chemistry, SB RAS Novosibirsk, Russia, chernyak@nioch.nsc.ru
7Novosibirsk Institute of Organic Chemistry, SB RAS Novosibirsk, Russia, dmitry@nioch.nsc.ru
8ICG SB RAS, Novosibirsk, Russia NSU, Novosibirsk, Russia, vikhorev@bionet.nsc.ru
9ICG SB RAS, Novosibirsk, Russia, shmakov@bionet.nsc.ru
10ICG SB RAS, Novosibirsk, Russia NSU, Novosibirsk, Russia, ak@bionet.nsc.ru
11Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Germany, kumlehn@ipk-gatersleben.de
12ICG SB RAS, Novosibirsk, Russia NSU, Novosibirsk, Russia Vavilov Institute of Plant Genetic Resources (VIR) Saint Petersburg, Russia, khlest@bionet.nsc.ru

Site-directed mutagenesis provides ample opportunity for reverse genetics, which allows us to establish a relationship between a gene and its function. In the present study, the role of the HvWIN1 barley (Hordeum vulgare) gene in the formation of epicuticular wax was identified by taking a targeted knockout approach using RNA-guided Cas9 endonuclease technology.

Bioinformatics and systems biology of plants symposiumComplete sequencing of barley organellar genomes: new data for intraspecific differentiation

Complete sequencing of barley organellar genomes: new data for intraspecific differentiation

No Comment on Complete sequencing of barley organellar genomes: new data for intraspecific differentiation

Poster (download)

[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/392.pdf”]
Yermakovich (Makarevich) Anna1, Siniauskaya Maryna2, Liaudanski Aleh3, Halayenka Innesa4, Davydenko Oleg5
1Institute of Genetics and Cytology of NAS of Belarus, Laboratory of Cytoplasmic Inheritance, bio.makarevich@gmail.com
2Institute of Genetics and Cytology of NAS of Belarus, Laboratory of Cytoplasmic Inheritance, m.sin@inbox.ru
3Institute of Genetics and Cytology of NAS of Belarus, Laboratory of Cytoplasmic Inheritance, 666555@tut.by
4Institute of Genetics and Cytology of NAS of Belarus, Laboratory of Cytoplasmic Inheritance, goloenkoi@tut.by
5Institute of Genetics and Cytology of NAS of Belarus, Laboratory of Cytoplasmic Inheritance, davydenko@tut.by

Organelle genomes are an important tool to investigate domestication, distribution and microevolution of plant species. However, they have found limited use in cereal intraspecific studies so far.

In the present study, organelle genomes of wild (Hordeum vulgare subsp. spontaneum) and cultivated (H. vulgare subsp. vulgare) barley forms were sequenced. We conducted theВ NGS of isolated chloroplast and mitochondrial DNA mixtures. This non-trivial approach allowed to obtain both genomes for each sample but required some specific steps in the data processing.

Comparative analysis of obtained sequences revealed more than 100 polymorphic sites in the chloroplast genome, including new intraspecific SSR-markers, and more than 20 polymorphisms in the mitochondrial genome.

We also carry out the phylogenetic analysis of these genomes. Chloroplast and mitochondrial DNA trees were consistent with each other, indicating the presence of two large clades containing both wild and cultivated forms.

Our results are conforming with a hypothesis of several domestication centres of barley. They also provide direct evidence of a higher rate of nucleotide substitutions in the chloroplast genomes as compared to that of mitochondria on a microevolution scale. The revealed high level of variability of chloroplast genomes makes it possible to use them for intraspecific barley differentiation.