International Multiconference “Bioinformatics of Genome Regulation and Structure\Systems Biology”

Tag

cytotoxicity

2 RESULTS
Systems Biology and Biomedicine symposiumCytotoxicity of Оі-Aluminum-silica-single-wall Carbon Nanotubes Carriers on Mesenchymal Stem Cells and Endothelial Cell Line EA.hy 926

Cytotoxicity of Оі-Aluminum-silica-single-wall Carbon Nanotubes Carriers on Mesenchymal Stem Cells and Endothelial Cell Line EA.hy 926

No Comment on Cytotoxicity of Оі-Aluminum-silica-single-wall Carbon Nanotubes Carriers on Mesenchymal Stem Cells and Endothelial Cell Line EA.hy 926

Poster (download)

[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/48.pdf”]
Irina Kim1, Alexander Lykov2, Lubov Rachkovskaya3, Maria Surovtseva4, Edmund Rachkovsky5, Olga Poveshchenko6
1Research Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, kii5@yandex.ru
2Research Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, aplykov2@mail.ru
3Research Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, noolit@niikel.ru
4Research Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, mfelde@ngs.ru
5Research Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, reed@academ.org
6Research Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics SB RAS Novosibirsk, Russia , Meshalkin National Medical Research Center, Ministry of Health of Russian Federation Novosibirsk, Russia, poveschenkoov@yandex.ru

In this study, Оі-aluminum-silica-single-wall carbon nanotubes carriers were synthesized and confirmed by scanning electron microscope, emission spectrometry, spectrophotometry. In the in vitro assays, the cytotoxicity on human bone marrow mesenchymal stem cells, and endothelial cell line EA.hy 926 were investigated by MTT assay, Griess reagent, and mixture of benzidine and hydrogen peroxide. The results of the proliferation, nitrogen production and intracellular myeloperoxidase activity showed that the Оі-aluminum-silica-single-wall carbon nanotubes carriers did not have a pronounced toxic effect on human cells. In conclusion, our results confirm that composition of Оі-aluminum-silica-single-wall carbon nanotubes may be used as carriers.

Systems Biology and Biomedicine symposiumCytotoxicity of Titanium Oxynitride Obtained By Reactive Magnetron Sputtered On Endothelial Cells Line EaHy 926

Cytotoxicity of Titanium Oxynitride Obtained By Reactive Magnetron Sputtered On Endothelial Cells Line EaHy 926

No Comment on Cytotoxicity of Titanium Oxynitride Obtained By Reactive Magnetron Sputtered On Endothelial Cells Line EaHy 926

Poster (download)

[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/158.pdf”]
Maria Surovtseva1, Olga Poveshchenko2, Alexander Lykov3, Irina Kim4, Vladimir Pichugin5, Irina Zhuravleva6
1Research Institute of Clinical ans Experimental Lymphology- Branch of the Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, mfelde@ngs.ru
2E. Meshalkin National Medical Research Center of the RF Ministry of Health Novosibirsk, Russia, poveschenkoov@yandex.ru
3Research Institute of Clinical ans Experimental Lymphology- Branch of the Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, aplykov2@mail.ru
4Research Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, kii5@yandex.ru
5Tomsk Polytechnic University Tomsk, Russia, pichuginvf@mail.ru
6E. Meshalkin National Medical Research Center of the RF Ministry of Health Novosibirsk, Russia, juravl_irina@mail.ru

AbstractOne of the current areas of medical materials science is the formation of ultrathin coatings on the surface of implants. Among the many types of coatings used in vascular surgery, binary and ternary titanium compounds have good prospects due to their high bio- and hemocompatibility. The properties of the coating based on films of nitrogen-containing titanium oxide (titanium oxynitride), applied, in particular, by reactive magnetron sputtering (RMS), was studied. While, problems of establishing and maintaining the necessary spraying mode is very relevant for the RMS technique. The aim of the study was determine the cytotoxicity of titanium oxy nitride compounds obtained under various modes of functioning of RMS on endothelial cells. It was showed that the levels of nitric oxide (NO) production by EaHy 926 cell line on Day 1 and Day 5 growth under the different samples of nitinol treated with different RMS modes was comparable to control. It was no obtained cytotoxic effect of the nitrogen-containing coatings of titanium oxide obtained by various modes of RMS on EaHy 926 cell line. While, was observed reduced levels of NO production by EaHy 926 cell line on Day 3.