Dysbiosis in the Gut Microbiota of Adolescents with Obesity
Novikova Evgenia Anatolyevna1, Bairova Tatyana Ananyevna2, Belkova Natalia Leonidovna3, Pogodina Anna Valeryevna4, Romanitsa Anastasia Igorevna5, Rychkova Lyubov Vladimirovna6
1Scientific РЎentre of Family Health and Human Reproduction Problems, europe411@mail.ru
2Scientific РЎentre of Family Health and Human Reproduction Problems, tbairova38@mail.ru
3Scientific РЎentre of Family Health and Human Reproduction Problems, nlbelkova@gmail.com
4Scientific РЎentre of Family Health and Human Reproduction Problems, pogodina_av@inbox.ru
5Scientific РЎentre of Family Health and Human Reproduction Problems, f1693@rambler.ru
6Scientific РЎentre of Family Health and Human Reproduction Problems, rychkova.nc@gmail.com
Gut microbiota plays a fundamental role in the pathogenesis of metabolic disorders including obesity. Gut microbial dysbiosis induces immune and metabolic disturbances. We wanted to find out a gut microbiota composition at adolescents with obesity and normal weight. The examined group included 40 adolescents. There were 18 obese adolescents with SDS BMI=2,77В±0,55 in the main group, and 22 adolescents with normal weight (SDS BMI=0,01В±0,50) in the control group. The metagenome sequencing of V3-V4 variable regions of 16S rDNA was done by Novogene Company (China).В Data were analyzed using the StatSoft STATISTICA 10.0 software package. Statistical significance was accepted at the p<0.05 level. We did not find any variations between two groups in the main phylum Firmicutes, Bacteroidetes, Proteobacteria, Actinobacteria, and following genera Bacteroides, Alistipes, Subdoligranulum, Megasphaera, Blautia, Akkermansia, Odoribacter, Lactobacillus, Bifidobacterium. But for phylotypes Enterobacter and Anaerotruncus the differences in gut microbiota composition were found. The obese participants had a 2-fold decrease in Enterobacter (p=0.02), and an increase – in the Anaerotruncus phylotype (p=0.04). Summing up the results we might say that adolescents with obesity had decreased the number of the phylum Proteobacteria (the Enterobacter phylotype) and increased the level of the phylum Firmicutes (the Anaerotruncus phylotype).