New insight on diversity of the Nikita Botanical Gardens plant collections from advanced NGS
Irina Mitrifanova1, Olga Krivenko2, Olga Kuleshova3, Svetlana Chelombit4, Valentina Brailko5, Olga Mitrofanova6
1NBG-NSC RAS, Yalta, Russia, irimitrofanova@yandex.ru
2NBG-NSC RAS, Yalta, Russia, olkrivenko@gmail.com
3NBG-NSC RAS, Yalta, Russia, v_olgo4ka@inbox.ru
4NBG-NSC RAS, Yalta, Russia, chelombit@inbox.ru
5NBG-NSC RAS, Yalta, Russia, labgennbs@yandex.ru
6NBG-NSC RAS, Yalta, Russia, invitro_plant@yandex.ru
New scientific program of genomic screening of the Nikita Botanical Gardens plant collections targeted at comprehensive description of their molecular diversity in close connection with morphological, physiological and others phenotype features has started. Strategic goal is to develop and implement advanced molecular approaches for plant biotechnology, breeding, propagation, and conservation routine works. Two whole-genome sequencing projects to produce a high quality assembly of the reference genomes of the common fig Ficus carica L. (Moraceae) and Crimean endemic species Heracleum ligusticifolium M. Bieb. (Apiaceae) are in progress. Several hybrid assembly approaches using combined data sets with long and short reads obtained by Oxford Nanopore MinION and Illumina NextSeq550 instruments are tested to create accurate and well-resolved reference genome. The first versions of the reference genomes of F. carica cultivar вЂSabrutsiya Rozovaya’ and H. ligusticifolium were made, and their annotations are performing. Subsequent improvement and development of the primary genome assemblies up to \”pan-genome\” rank will be carried out using advanced bioinformation approaches and computational tools by assimilation of new sequenced genomes and transcriptomes of the model species taken from the NBS-NSC collections and from nature biotopes as well. Such integrative reference genome construction will provide an important resource both for advanced molecular breeding and fundamental researches in plant biology and ecology based on genomic approaches using F. carica and H. ligusticifolium as model species.