Poster (download)
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Nina Illarionova1, Maria Borisova2, Ekaterina Bazhenova3, Daria Fursenko4, Daria Zabelina5, Nikita Khotskin6, Alexander Kulikov7
1ICG SB RAS, Novosibirsk, Russia, nina.illarionova@gmail.com
2ICG SB RAS, Novosibirsk, Russia, zolotykh@bionet.nsc.ru
3ICG SB RAS, Novosibirsk, Russia, ekaterina.yu.bazhenova@gmail.com
4Institute of Gene Biology RAS, Moscow, Russia, cenzoored@gmail.com
5NSU, Novosibirsk, Russia, dazabelina@gmail.com
6ICG SB RAS, Novosibirsk, Russia, khotskin@bionet.nsc.ru
7ICG SB RAS, Novosibirsk, Russia, v_kulikov@bionet.nsc.ru
Kaiso is a transcription factor that is known to regulate cell division in different cell types. Kaiso binds kaiso specific binding site and/or methylated CpG islands on the promoter region of different target genes. Kaiso silencing may lead to an increase or a decrease in cell proliferation, which is cell specific and is probably dependent on the methylation status of Kaiso binding sites at the target promoter. In various mammalian cell types Kaiso was shown to regulate cell proliferation via its target genes of cyclins E1 and D1.В Kaiso is widely expressed throughout the brain regions, however its regulation of cell division in brain development was not investigated.В Our preliminary data showed that in the developing hippocampusВ there was a significant increase in the number of newborn cells in KO mice (17 В± 1) compared with the WT mice (12 В± 1 per 100 Ојm2; p <0,001). The number of newborn cells co-stained with the neuron marker was also significantly greater in KO mice (6 В± 1) compared with the WT mice (4 В± 1 per 100 Ојm2; p <0,05).В In the cortex, striatum, and subventricular zone of the lateral ventricles, there were no significant differences between genotypes in the number of newborn cells.В We have performed expression profiling of genes associated with cell division regulation (c-myc, CCNE1 and CCND1) that were previously noted to be regulated by Kaiso in different cell types. A significant decrease in the expression of the c-myc gene was shown in the hippocampus of KO mice at the age P2 compared to WT (KO: 2,7 В± 0,3; WT: 4,5 В± 0,7; p <0,05). On the mRNA level neither CCNE1 nor CCND1 expression was different between the genotypes in all selected brain regions and corresponding age groups.В Supported by the RFBR (18-04-00869 Рђ)
