Mechanisms of ischemic kidney tolerance in young and senescence-accelerated rats exposured to ischemic preconditioning or calorie restriction
Egor Plotnikov1, Nadezda Andrianova2, Stanislovas Jankauskas3, Irina Pevzner4, Ljubava Zorova5, Vasily Popkov6, Denis Silachev7, Natalia Kolosova8, Dmitry Zorov9
1A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, plotnikov@belozersky.msu.ru
2A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, andnadya12@gmail.com
3A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, jankauskas.ss@gmail.com
4A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, irinapevzner@mail.ru
5A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, lju_2003@list.ru
6A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, popkov.vas@gmail.com
7A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, silachev_dn@belozersky.msu.ru
8Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (SB RAS), kolosova@bionet.nsc.ru
9A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, zorov@belozersky.msu.ru
Dietary restriction and ischemic preconditioning are the most efficient approaches ameliorating the severity of different pathological conditions. We investigated the protective potential of long-term calorie restriction and short ischemic preconditioning protocol in the model of acute kidney injury (AKI) in young Wistar and OXYS rats. In young rats, ischemic preconditioning, which consists of 4 cycles of ischemia and reperfusion alleviated kidney injury caused by 40-min ischemia. However, 6-month-old OXYS rats having signs of premature aging lost their ability to protect the ischemic kidney by IPC. However, CR of OXYS rats led to a significant decrease in creatinine and BUN levels after kidney ischemia, indicating significant protection.