Analysis of sequenced chromosome-specific libraries of gekkonids sheds light to large scale genome reshuffling in reptiles
Katerina Tishakova1, Dmitry Prokopov2, Ilya Kichigin3, Anna Molodtseva4, LukГЎЕЎ Kratochvil5, Artem Lisachov6, Vladimir Trifonov7
1Novosibirs State University, Novosibirsk, Russia; Institute of Molecular and Cellular Biology SB RAS, Novosibirsk, Russia, k.tishakova@g.nsu.ru
2Institute of Molecular and Cellular Biology SB RAS, Novosibirsk, Russia, dprokopov@mcb.nsc.ru
3Institute of Molecular and Cellular Biology SB RAS, Novosibirsk, Russia, kig@mcb.nsc.ru
4Institute of Molecular and Cellular Biology SB RAS, Novosibirsk, Russia, rada@mcb.nsc.ru
5Charles University, Prague, Czech Republic, lukkrat@email.cz
6Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia, lisachov@bionet.nsc.ru
7Novosibirs State University, Novosibirsk, Russia; Institute of Molecular and Cellular Biology SB RAS, Novosibirsk, Russia, vlad@mcb.nsc.ru
Representatives of Infraorder Gekkota demonstrate relatively low chromosomal evolution and rapidly evolved sex chromosome systems. Using low-coverage chromosome sequencing, we sequenced chromosome-specific libraries of Gekko japonicus and putative sex chromosome of Hemidactylus platyurus to compare with annotated genomes of squamate reptiles. We found evolutionary long conservation of macro-chromosome and high dynamic of micro-chromosomes. Also, we discovered homologues synteny blocks between sex chromosomes of species from different infraorder.