International Multiconference “Bioinformatics of Genome Regulation and Structure\Systems Biology”

Tag

mesenchymal stem cells

2 RESULTS
Systems Biology and Biomedicine symposiumHuman Dermal Fibroblasts and Bone-Marrow Mesenchymal Stem Cells properties under Silver and Lithium Condition

Human Dermal Fibroblasts and Bone-Marrow Mesenchymal Stem Cells properties under Silver and Lithium Condition

No Comment on Human Dermal Fibroblasts and Bone-Marrow Mesenchymal Stem Cells properties under Silver and Lithium Condition

Poster (download)

[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/12.pdf”]
Alexander Lykov1, Lubov Rachkovskaya2, Olga Poveshchenko3, Maria Surovtseva4, Irina Kim5, Edmund Rachkovsky6, Alena Philippova7
1Research Institute of Clinical ans Experimental Lymphology- Branch of the Institute of Cytology and Genetics SB RAS, aplykov2@mail.ru
2Research Institute of Clinical ans Experimental Lymphology- Branch of the Institute of Cytology and Genetics SB RAS, lymphology@niikel.ru
3Research Institute of Clinical ans Experimental Lymphology- Branch of the Institute of Cytology and Genetics SB RAS, poveschenkoov@yandex.ru
4Research Institute of Clinical ans Experimental Lymphology- Branch of the Institute of Cytology and Genetics SB RAS, mfelde@ngs.ru
5Research Institute of Clinical ans Experimental Lymphology- Branch of the Institute of Cytology and Genetics SB RAS, kii5@yandex.ru
6Research Institute of Clinical ans Experimental Lymphology- Branch of the Institute of Cytology and Genetics SB RAS, lymphology@niikel.ru
7Municipal autonomous educational institution Education center Gornostay, alena.philippova2003@yandex.ru

Fibroblasts and mesenchymal stem cells are involved in damaged skin repair. Functional activity of the fibroblasts mesenchymal stem cells depends from environment, including antimicrobial preparations. Silver salts are one of the antimicrobial substances. Lithium salts exhibit not only a stabilizing effect on human mental responses, but also antitumor and autophagic effects. The “Bodyguard” hygienic composition contains both silver and lithium. The aim of the study was to study the effect of bound silver and lithium ions in the \”Bodyguard\” composition and in salt of silver nitrate and lithium of citric acid on human dermal fibroblasts (DFs) and bone-marrow mesenchymal stem cells (MSCs) functions. It has been shown that DFs in the presence of \”Bodyguard\” reduces proliferative activity and significantly increases care in apoptosis. At the same time, silver and lithium ions alone or in combination do not affect proliferation, enhance migration and reduce DFs apoptosis. It has been shown that MSCs proliferation increased under “Bodyguard” condition. Whereas, silver ions and lithium ions not have any effect on proliferation of MSCs, but influenced on myeloperoxidase activity, nitric oxide production, migration, and apoptosis. The more pronounced negative action of the “Bodyguard” is most likely due not to silver and lithium ions, but to the very structure of the carrier to which these ions are immobilized.

Systems Biology and Biomedicine symposiumProteomic Analysis of Extracellular Vesicles Produced by Placental Mesenchymal Stem Cells

Proteomic Analysis of Extracellular Vesicles Produced by Placental Mesenchymal Stem Cells

No Comment on Proteomic Analysis of Extracellular Vesicles Produced by Placental Mesenchymal Stem Cells

Anastasia Khutornenko1, Anastasia Zharikova2, Vasily Popkov3, Sergey Kovalchuk4, Kirill Goryunov5, Yulia Shevtsova6, Egor Plotnikov7, Dmitry Zorov8, Denis Silachev9
1Laboratory of Cell Technologies, Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia, bioingenier@gmail.com
2Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russia, azharikova89@gmail.com
3The A.N. Belozersky Institute Of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia, popkov.vas@gmail.com
4Laboratory of Bioinformatic methods for Combinatorial Chemistry and Biology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia, xerx222@gmail.com
5Laboratory of Cell Technologies, Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia, kirishgor@gmail.com
6Laboratory of Cell Technologies, Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russia, yulshevtsova@yandex.ru
7A.N. Belozersky Institute of Physico-Chemical Biology; Research Center of Obstetrics, Gynecology and Perinatology, Moscow, Russia, plotnikov@belozersky.msu.ru
8A.N. Belozersky Institute of Physico-Chemical Biology; Reserch Center of Obstetrics, Gynecology and Perinatology, Moscow, Russia, zorov@belozersky.msu.ru
9A.N. Belozersky Institute of Physico-Chemical Biology; Research Center of Obstetrics, Gynecology and Perinatology, Moscow, Russia, d_silachev@oparina4.ru

Mesenchymal stem/stromal cells (MSCs) have therapeutic potential in many pathological conditions, that is explained mostly by their paracrine action. MSCs secrete extracellular vesicles (EVs) packed with proteins, DNA, RNA and lipids. One of the easily accessible and reach sources of MSCs is placenta. In order to reveal possible pathways, by which EVs produced by placenta-derived MSCs (EVs-PMSC) could realize their therapeutic potential we conducted proteomic analysis of EVs-PMSC and subsequent bioinformatic analysis of identified proteins using Reactome pathway database. Identified peptides were mapped to 2093 proteins. Among the 2093 proteins, 972 proteins were identified in at least four LC-MS/MS analyses out of eight. According to Reactome the most significant ‘top three’ 1st level pathways for detected EVs-PMSC proteins were ‘Cellular responses to external stimuli’; ‘Immune system’; ‘Developmental biology’. Some of the most enriched pathways in the group ‘Cellular responses to external stimuli’ were: ‘Response of EIF2AK4 (GCN2) to amino acid deficiency’; ‘Cellular response to hypoxia’; ‘Detoxification of Reactive Oxygen Species’. Some of the most enriched pathways in the group ‘Immune System’ were: ‘Class I MHC mediated antigen processing & presentation’; ‘Neutrophil degranulation’; ‘Interferon Signaling’. The most enriched pathway in the group ‘Developmental biology’ was ‘Axon guidance’. Thus far, concerning most enriched pathways EVs-PMSC proteome is expected to: 1) provide protection against stress (amino acid deficiency, hypoxia, ROS) to the damaged tissues; 2) possess immunomodulation properties; 3) facilitate axon guidance. The result (the top list of enriched pathways) leads to the notion that EVs-PMSC could be effective in terms of neuroprotection.