International Multiconference “Bioinformatics of Genome Regulation and Structure\Systems Biology”

Tag

microRNA

3 RESULTS
Systems Biology and Biomedicine symposiumMICRORNAS (221, 429) ARE CORRELATION WITH LYMPHOCYTES OF AXILLARY LYMPH NODES IN EXPERIMENTAL BREAST CANCER

MICRORNAS (221, 429) ARE CORRELATION WITH LYMPHOCYTES OF AXILLARY LYMPH NODES IN EXPERIMENTAL BREAST CANCER

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Poster (download)

[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/17.pdf”]
Aleksey Kabakov1, Alexandr Lykov2, Oleg Kazakov3, Alexandr Poveshchenko4, Olga Poveshchenko5, Dmitriy Strunkin6, Lyudmila Gulyaeva7, Andrey Letyagin8, Vladimir Konenkov9
1RICEL-Branch of IC&G SB RAS, kabakov_av@mail.ru
2RICEL-Branch of IC&G SB RAS, aplykov2@mail.ru
3RICEL-Branch of IC&G SB RAS, kazakoff_oleg@mail.ru
4RICEL-Branch of IC&G SB RAS, poveshchenkoa200@mail.ru
5RICEL-Branch of IC&G SB RAS, poveschenkoov@yandex.ru
6RIMBB FRC FTM, strunkind@mail.ru
7RIMBB FRC FTM, gulyaeva@soramn.ru
8RICEL-Branch of IC&G SB RAS, letyaginay@bionet.nsc.ru
9RICEL-Branch of IC&G SB RAS, vikonenkov@gmail.com

The study of concentration miRNA (21, 221, 222, and 429) in serum, lymph of the thoracic duct and breast cancer tissue, as well as the cellular composition of axillary lymph node in Wistar female rats in chemically induced breast cancer. Concentration microRNA (-221, -429) in the tissues breast cancer and lymph are correlated with a decrease in number of lymphocytes in medullary cords of axillary lymph nodes.

Systems computational biology: analysis, mathematical modeling and information technologies symposiumAn architecture-independent algorithm for microRNA target prediction

An architecture-independent algorithm for microRNA target prediction

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Natalya Fokina1, Alexander Grinev2
1Moscow State Medical University, retrospector@ispras.ru
2Moscow State Medical University, grinev.ab@1msmu.ru

Nowadays the research on RNA interference is a subject of growing interest. One of the most perspective directions of this research is the use of bioinformatics methods to predict possible interactions of microRNAs with their mRNA counterparts, which allows to increase the accuracy and efficiency of their confirmation using laboratory means. In spite of this fact, the existing tools are unable to meet all the needs of molecular biology. An example of a problem currently lacking an appropriate solution is the study of RNA interference between organisms belonging to different taxonomic groups, notably, in parasite-host systems. Parasites utilise the host cell as a source of nutrients and habitat; molecular genetic profiles of both participants of this interaction are subject to dynamic changes. In this paper we present a novel algorithm for detection of interactions involving RNA molecules in a wide range of organisms. The proposed solution exceeds the existing ones in several characteristics and, in our view, can be broadly used in molecular biology and bioinformatics.

Genomics, transcriptomics, bioinformatics symposiumMicroRNA content of horse and human milk exosomes

MicroRNA content of horse and human milk exosomes

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Sergey Sedykh1, Kuleshova Anna2, Georgy Nevinsky3
1SB RAS ICBFM, NSU, sedyh@niboch.nsc.ru
2SB RAS ICBFM, aekuleshova25@gmail.com
3SB RAS ICBFM, NSU, nevinsky@niboch.nsc.ru

Exosomes are 40-100 nm diameter natural vesicles, containing CD9, CD63 and CD81 tetraspanins on the surface. It was shown that milk obtained from different sources contain exosomes: human, horse, bovine, rat. Horse milk is a unique source of exosomes since is less allergenic than bovine and not prion prone. Also, horse milk can be obtained in larger amounts than human milk.

According to the results, published by the other research groups, milk exosomes contain hundreds and thousands of proteins, mRNA and microRNA molecules. Our recent results show that these numbers may be significantly overestimated.В Isolation of exosomes from sediments obtained after ultracentrifugation with an additional step of gel-filtration
allows decreasing the number of proteins, that co-isolate with vesicles. Many microRNAs are described in exosomes, obtained from human and bovine milk. Here we show the content of more than two dozens major microRNA molecules, isolated fromВ human and horse milk exosomes on different stages of centrifugations, ultracentrifugations, before and after gel-filtration. According to these data, several microRNAs may be used as indicators of milk exosomes\’ purity.

The study was funded by the Russian Scientific Foundation (research project 187410055 to S. Sedykh) and by the Program of Fundamental Research of Government Academia (РђРђРђРђ-Рђ17-117020210023-1 to Prof. G. Nevinsky).