International Multiconference “Bioinformatics of Genome Regulation and Structure\Systems Biology”

Tag

retrotranscriptome

2 RESULTS
Bioinformatics and systems biology of plants symposiumRetrotransposons of Arabidopsis thaliana expressed in wild-type plants

Retrotransposons of Arabidopsis thaliana expressed in wild-type plants

No Comment on Retrotransposons of Arabidopsis thaliana expressed in wild-type plants

Sofya Gvaramiya1, Murad Omarov2, Ilya Kirov3
1Department of marker-assisted and genomic selection of plant All-Russia Research Institute of Agricultural Biotechnology, sofia.gvaramia@gmail.com
2Department of marker-assisted and genomic selection of plant All-Russia Research Institute of Agricultural Biotechnology, muradok98@gmail.com
3Department of marker-assisted and genomic selection of plant All-Russia Research Institute of Agricultural Biotechnology, kirovez@gmail.com

Mobile genetic elements are widely spread across the genomes of plants and animals. Of them, retrotransposons occupy substantial part of the genome. They had a huge impact on host genome evolution. However, their current activity in plant cell is thought to be very low on both RNA and cDNA (mobilome) levels because multiple silencing pathways. To get deeper insight into the mechanisms of mobilome formation we carried out identification of transcribed TEs of model plant, Arabidopsis thaliana. Using bioinformatics and ‘wet’ approaches we found evidence of expression of 6 TEs of A.thaliana. This opens a door for further functional studies of how some TEs can overcome the genome defense against retrotransposon activity.

Genomics, transcriptomics, bioinformatics symposiumLTR-retrotransposon transcripts are ubiqutiously expressed, polyadenylated and underwent splicing in sunflower (Helianthus annuus L.)

LTR-retrotransposon transcripts are ubiqutiously expressed, polyadenylated and underwent splicing in sunflower (Helianthus annuus L.)

No Comment on LTR-retrotransposon transcripts are ubiqutiously expressed, polyadenylated and underwent splicing in sunflower (Helianthus annuus L.)

Pavel Merkulov, Murad Omarov, Ilya Kirov1
1All-Russian Research Institute of Agricultural Biotechnology RAS, paulmerkulov97@gmail.com

LTR-retrotransposons are mobile genetic elements widespread in plant genomes. Dispite the fact of significant part of genome engaged by retrotransposons (up to 80 percent), their activity is suppressed by multiple posttranscriptional and epigenetic mechanisms in normal conditions. Retrotransposon escape from silencing and subsequent expression are, in turn, purely described for plants.