International Multiconference “Bioinformatics of Genome Regulation and Structure\Systems Biology”

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small-incision lenticule extraction

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Systems Biology and Biomedicine symposiumReLEx SMILE Method to Expansion and Create Scaffolds from Corneal Fibroblasts

ReLEx SMILE Method to Expansion and Create Scaffolds from Corneal Fibroblasts

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Poster (download)

[pdf-embedder url=”https://bgrssb.icgbio.ru/wp-content/uploads/2020/07/83.pdf”]
Kristina Krasner1, Alexander Lykov2, Olga Poveshchenko3, Valeriy Chernykh4, Maria Surovtseva5, Irina Kim6
1S. Fedorov Eye Microsurgery Federal State Institution Novosibirsk, Russia, kityli@mail.ru
2Research Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, aplykov2@mail.ru
3Research Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, poveschenkoov@yandex.ru
4S. Fedorov Eye Microsurgery Federal State Institution Novosibirsk, Russia, rimma@mntk.nsk.ru
5Research Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, mfelde@ngs.ru
6Research Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics SB RAS Novosibirsk, Russia, kii5@yandex.ru

To identify three-dimensional corneal stromal constructs that could promote a human corneal fibroblasts survival. Small-incision lenticule extraction was used for obtained corneal stroma for in vitro expansion of primary human corneal fibroblasts; monoclonal antibodies to keratocyte, mesenchymal stem cell/fibroblast and epithelial cell was used to phenotyping expanded human corneal fibroblast on the flow cytometry; polyethylene glycol, platelet lysate and methylene cellulose was used to prepare three-dimensional corneal stroma constructs, morphology was monitored by phase contrast microscopy. During 3-4 weeks human corneal fibroblasts was expanded in vitro from lenticule. Obtained cells expressed mesenchymal stem cell/fibroblast surface marker and was negative for keratocyte and epithelial cells surface markers. A combination of polyethylene glycol with platelet lysate significantly increased growth and survival of human corneal fibroblasts in three-dimensional corneal stroma construct.