International Multiconference “Bioinformatics of Genome Regulation and Structure\Systems Biology”

Tag

transcriptome

2 RESULTS
Bioinformatics and systems biology of plants symposiumSTUDY OF THE ROOT TRANSCRIPTOME OF BREAD WHEAT USING HIGH-THROUGHPUT RNA SEQUENCING (RNA-SEQ)

STUDY OF THE ROOT TRANSCRIPTOME OF BREAD WHEAT USING HIGH-THROUGHPUT RNA SEQUENCING (RNA-SEQ)

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Alexandr Vikhorev1, Nikolay Shmakov2, Anastasia Glagoleva3, Elena Khlestkina4, Olesya Shoeva5
1Novosibirsk State University, vikhorev@bionet.nsc.ru
2Institute of Cytology and Genetics SB RAS, shmakov@bionet.nsc.ru
3Institute of Cytology and Genetics SB RAS, glagoleva@bionet.nsc.ru
4All-Russian Institute of Plant Recources, khlest@bionet.nsc.ru
5Institute of Cytology and Genetics SB RAS, olesya_ter@bionet.nsc.ru

Bread wheat (Triticum aestivum L.) is the most important crop in the world. It provides about 20% of the total calories consumed by humans. For a long time, wheat selection was mainly based on phenotypic traits of the shoot, but the roots were given little attention. As a result, the root system of modern wheat varieties has weakened. Therefore, the study of genetic control of wheat roots development is an urgent issue. In this study, sequencing of RNA libraries from roots and coleoptile of Russian spring variety “Saratovskaya, 29” was performed. De novo transcriptome was assembled. 31,488 up-regulated, 35,851 down-regulated and 18,040 roots-specific transcripts were found. The subsequent analysis of genes with differential expression will allow choosing the candidate genes for development of wheat varieties with resistant root system.

Genomics, transcriptomics, bioinformatics symposiumFunctioning of unique nitrile-detoxifying system in soil xenobiotic degrader Rhodococcus rhodochrous: a whole-genome transcriptomic approach

Functioning of unique nitrile-detoxifying system in soil xenobiotic degrader Rhodococcus rhodochrous: a whole-genome transcriptomic approach

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Konstantin V Lavrov1, Andrey D Novikov2, Tatyana I Kalinina3, Artem S Kasianov4, Alexander S Yanenko5
1NRC “Kurchatov institute – GosNIIgenetika”, lavrov.ko@gmail.com
2NRC “Kurchatov institute – GosNIIgenetika”, alexm19@mail.ru
3NRC “Kurchatov institute – GosNIIgenetika”, tatyana.i.kalinina@googlemail.com
4Vavilov Institute of General Genetics, artem.kasianov@gmail.com
5NRC “Kurchatov institute – GosNIIgenetika”, yanenko@genetika.ru

Organic nitriles are significant participants of interactions between soil living organisms, thus their synthesis and degradation are of interest. Using transcriptomic approach, we revealed a genetic cascade, realizing nitrile degradation in powerful nitrile degrader – Rhodococcus rhodochrous. We also hypothesized a genetic “network” for nitrile/amide utilization, connected with cobalt homeostasis.