Accepted_test

Comparative genomic studies in three duck’s species (the Northern Pintail, Mallard, and Common Goldeneye) and Karyotype Evolution in the Family Anatidae (Anseriformes, Aves)
Authors:
Proskuryakova Anastasia, Institute of Molecular and Cellular Biology, Siberian Branch of Russian Academy of Sciences (SB RAS), Novosibirsk, Russian Federation
Beklemisheva Violetta, Institute of Molecular and Cellular Biology, Siberian Branch of Russian Academy of Sciences (SB RAS), Novosibirsk, Russian Federation
Tishakova Katerina, Institute of Molecular and Cellular Biology, Siberian Branch of Russian Academy of Sciences (SB RAS), Novosibirsk, Russian Federation
Romanenko Svetlana, Institute of Molecular and Cellular Biology, Siberian Branch of Russian Academy of Sciences (SB RAS), Novosibirsk, Russian Federation
Andreushkova Daria, Institute of Molecular and Cellular Biology, Siberian Branch of Russian Academy of Sciences (SB RAS), Novosibirsk, Russian Federation
Yudkin Vladimir, Institute of Systematics and Ecology of Animals, SB RAS, Novosibirsk, Russian Federation; Novosibirsk State University, Novosibirsk, Russian Federation
Interesova Elena, Institute of Systematics and Ecology of Animals, SB RAS, Novosibirsk, Russian Federation; Tomsk State University, Tomsk, Russian Federation
Yang Fengtang, School of Life Sciences and Medicine, Shandong University of Technology, Zibo, China
Ferguson-Smith Malcolm, Cambridge Resource Center for Comparative Genomics, University of Cambridge, Cambridge, UK
Graphodatsky Alexander, Institute of Molecular and Cellular Biology, Siberian Branch of Russian Academy of Sciences (SB RAS), Novosibirsk, Russian Federation
Abstract ID: 125
Event: BGRS-abstracts
Sections: [Sym 7] Section “Genomics, genetics and systems biology of animals”

Galliformes and Anseriformes represent two branches of the Galloanserae group. While Galliformes have been extensively studied, the evolution of Anseriformes karyotypes remains relatively understudied, with only a few species included in comparative genomic analyses. Therefore, this study aims to conduct comparative genomic analyses of the karyotypes of three representatives of the order Anseriformes from the Anatidae family: the mallard (Anas platyrhynchos), pintail (Anas acuta), and common goldeneye (Bucephala clangula). Analysis of the karyotypes were performed by routine staining and GTG, CBG, CDAG methods and revealed that all studied species possess the same diploid number of chromosomes (2n=80). However, the karyotype of the common goldeneye differs from those of representatives of the genus Anas due to an increased number of macrochromosomes and the presence of large blocks of heterochromatin on most macroautosomes and sex chromosomes. The utilization of stone curlew chromosome specific probes by fluorescence in situ hybridization revealed homologies on duck’s macro- and microchromosomes. Previous studies comparing chicken and stone curlew karyotypes facilitated the identification of homologies between chicken chromosomes and the karyotypes of the three duck species. Interspecific comparisons revealed that the increase in macrochromosome numbers in the common goldeneye karyotype is due to fissions in two ancestral elements. Clusters of rRNA genes were found on four pairs of pintail microchromosomes and on two pairs of common goldeneye microchromosomes. Our study demonstrated that Anatidae species possess microchromosomes enriched in telomeric sequences. All these findings contribute to understanding of the evolutionary transformations of macro- and sex chromosomes in Anseriformes.