Accepted_test

Promoters of genes encoding β-amylase, albumin, and globulin in food plants have weaker affinity for TATA-binding protein as compared to non-food plants: in silico analysis
Authors:
Podkolodnaya O., Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia
Chadaeva I., Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia
Zolotareva K., Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia
Podkolodnyy N., Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia
Khandaev B., Novosibirsk State University, Novosibirsk, Russia
Rasskazov D., Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia
Bogomolov A., Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia
Kazachek A., Novosibirsk State University, Novosibirsk, Russia
Vishnevsky O., Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia
Sharypova E., Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia
Ponomarenko P., Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia
Savinkova L., Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia
Zemlyanskaya E., Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia
Ponomarenko M., Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia
Abstract ID: 132
Event: BGRS-abstracts
Sections: [Sym 1] Section “Genome-wide transcriptomics (differential gene expression)”

Motivation and Aim: It is generally accepted that during the domestication of food plants, the selection was focused on their productivity, the ease of their technological processing into food, and resistance to pathogens and environmental stressors. Nonetheless, it is unclear whether in antiquity, aside from positive selection for beneficial properties of plants, humans also simultaneously selected against such detrimental properties as allergenicity.

Methods and Algorithms: Accordingly, in this paper, albumin, globulin, and β-amylase of common wheat Triticum aestivum L. (1753) are analyzed, which have been identified earlier as targets for attacks by human class E immunoglobulins. At the genomic level, we wanted to find signs of past negative selection against the allergenicity of these three proteins (albumin, globulin, and β-amylase) during the domestication of ancestral forms of modern food plants. Using our web service Plant_SNP_TATA_Z-tester, here we have estimated the equilibrium dissociation constant (KD) of TATA-binding protein (TBP) complexes with plant promoters using 90 bp of their DNA sequences of 235 genes representing 28 plant species.

Results: It was found that compared with non-food plants, food plants are characterized by statistically significantly weaker affinity of TBP for proximal promoters of their genes homologous to the genes of common-wheat globulin, albumin, and β-amylase (food allergens): p < 0.01 according to Fisher's Z-test.

Conclusion: This suggests that in the past, humans to reduce the expression of food plant genes encoding these allergenic proteins carried out spontaneous artificial selection.