Accepted_test

Crystal structure of DNA with histone-like protein Dps complex

Authors:
Kovalenko Vladislav, N.N. Semenov Federal Research Center for Chemical Physics, RAS, Moscow, Russia
Loiko Natalia, Federal Research Center Fundumentals of Biotechnology, RAS, Moscow, Russia
Tereshkina Ksenia, N.N. Semenov Federal Research Center for Chemical Physics, RAS, Moscow, Russia
Tereshkin Eduard, N.N. Semenov Federal Research Center for Chemical Physics, RAS, Moscow, Russia
Krupyanskii Yuriy, N.N. Semenov Federal Research Center for Chemical Physics, RAS, Moscow, Russia

Abstract ID: 14

Event: BGRS-abstracts

Sections: [Sym 3] Section “Structural biology of proteins nucleic acids and membranes”

Our work is devoted to structure of crystalline DNA-Dps complex, which contained Dps protein in its altered quaternary structure. Atom positions of this quaternary structure were obtained from three monomers of known dodecameric structure of Dps protein, which was subjected to energy relaxation process during 1 μs in full atomic forcefield in Gromacs 5.1. After that Dps trimers and DNA molecules were arranged in experimental electron density of a crystal complexes obtained from TEM.