Accepted_test

Genetic and pharmacological piwi suppression modulates lifespan and radioresistance of Drosophila melanogaster

Authors:
Proshkina Ekaterina, Institute of Biology, FRC Komi SC UrB RAS, Syktyvkar, Russia
Ulyasheva Natalia, Institute of Biology, FRC Komi SC UrB RAS, Syktyvkar, Russia
Pakshina Natalya, Institute of Biology, FRC Komi SC UrB RAS, Syktyvkar, Russia
Koval Liubov, Institute of Biology, FRC Komi SC UrB RAS, Syktyvkar, Russia
Yushkova Elena, Institute of Biology, FRC Komi SC UrB RAS, Syktyvkar, Russia
Solovev Ilya, Institute of Biology, FRC Komi SC UrB RAS, Syktyvkar, Russia
Shaposhnikov Mikhail, Institute of Biology, FRC Komi SC UrB RAS, Syktyvkar, Russia
Moskalev Alexey, Institute of Biology, FRC Komi SC UrB RAS, Syktyvkar, Russia

Abstract ID: 300

Event: BGRS-abstracts

Sections: Section “Genetics and systems biology of aging”

We have studied the contribution of piwi gene activity in various imago tissues to the regulation of lifespan and radioresistance of Drosophila melanogaster. Knockdown of the piwi gene in the nervous system and fat body causes an increase in the lifespan of Drosophila and increases the activity of genes associated with stress-response and longevity. In this case, suppression of piwi in the fat body stimulates the resistance to γ-radiation, and neuronal knockdown reduces it. Thereafter, we have identified potential inhibitors of PIWI proteins. Five of them (atenolol, metoprolol, imipenem, fingolimod and misoprostol) have shown geroprotective properties. The most pronounced and reproducible increase in lifespan cause by metoprolol and imipenem in females. The effect of potential PIWI inhibitors on radioresistance is ambiguous and depends on the drug concentration and radiation dose.