Accepted_test

Search for the transcription factors involved in the regulation of biofilm formation in Escherichia coli K-12
Authors:
Tatiana Bessonova, Institute of Cell Biophysics of the Russian Academy of Sciences, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Pushchino, Russia ; Institute for Information Transmission Problems of the Russian Academy of Sciences (Kharkevich Institute), Moscow, Russia
Artem Dakhnovets, Skolkovo Institute of Science and Technology, Moscow, Russia
Uliana Kuznetsova, Pirogov Russian National Research Medical University, Moscow, Russia
Olga Ozoline, Institute of Cell Biophysics of the Russian Academy of Sciences, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Pushchino, Russia
Mikhail Gelfand, Skolkovo Institute of Science and Technology, Moscow, Russia
Maria Tutukina, Skolkovo Institute of Science and Technology, Moscow, Russia; nstitute for Information Transmission Problems of the Russian Academy of Sciences (Kharkevich Institute), Moscow, Russia; Institute of Cell Biophysics of the Russian Academy of Sciences, Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, Pushchino, Russia
Abstract ID: 513
Event: BGRS-abstracts
Sections: [Sym 1] Section “Regulatory genomics”

Васterial biofilms are one of the main reasons for the worldwide expanding antibiotic resistance. It takes 1,000 times as much antibiotic to treat an infection if bacteria “decide” to form a biofilm. Lots of studies are being conducted for Pseudomonas and Staphylococcus. However, the regulation of biofilm formation in Escherichia coli, which can also be pathogenic, is still poorly understood. Uropathogenic E. coli (UPEC), forming extensive biofilms, leads to chronic urinary tract infections, while the biofilms formed by EPEC can be the reason for chronic colitis or gastroenteritis. The laboratory opportunistic E. coli K-12 MG1655 strain is also characterized by biofilm formation. A variety of candidate regulators of this process have been proposed but the master regulator is not yet known. In this study we have shown that hexuronates metabolism and its regulators, UxuR and YjjM, play the key roles in successful bacterial attachment and biofilm formation via modulating these processes, while cAMP-CRP probably act indirectly. Also, our data suggest that small non-coding RNAs are deeply involved in regulation of biofilm formation in E. coli K-12 and might serve as possible signaling molecules.