Accepted_test

Nanopore sequencing of concatemeric libraries, with the derivation of monomeric sequence consensus, allows for the obtainment of sufficiently long reads with higher accuracy. We propose a new method for obtaining concatemeric libraries of amplicons without primers during the concatemer production stage by using rolling circle amplification (RCA) to decrease the portion of chimeric molecules. We conducted a preliminary test of the developed protocol. As the result, the entire length of the TCRA and TCRB variable regions were successfully reconstructed using UMI-labeled (UMI-Unique Molecular Identifiers) monomers. The initial data suggests that this approach is very promising for whole length sequencing and high throughput profiling of heterogeneous RNAs with high similarity, such as TCR, BCR, and retroelements in tumor samples.