Accepted_test

ACME HS dissociation: an adaptable cell fixation-dissociation method for fresh-frozen human adrenal gland tissues for scRNA-seq

Authors:
Anastasia Shcherbakova, The National Medical Research Center for Endocrinology
Marina Utkina, The National Medical Research Center for Endocrinology
Ruslan Deviatiiarov, Graduate School of Medicine, Juntendo University, Tokyo, Japan
Alina Ryabova, The National Medical Research Center for Endocrinology
Lilia Urusova, The National Medical Research Center for Endocrinology

Abstract ID: 236

Event: BGRS-abstracts

Sections: [Sym 1] Section “Genome-wide transcriptomics (differential gene expression)”

Sample preparation is a crucial step in single-cell RNA sequencing (scRNA-seq) of human tissues to obtain biologically relevant data. Current methods encounter challenges in obtaining high-quality single-cell suspensions, especially in tissues where live cell isolation is difficult. To address this gap, we introduce ACME HS (ACetic (acid)-MEthanol Hight Salt), an optimized approach that facilitates the retrieval of intact and whole cells from fresh-frozen tissues. In this study, we conducted a comparative analysis of cellular heterogeneity in datasets of single cells from the human adrenal medulla obtained from ACME HS, enzymatic dissociation, and nuclei isolation.