Accepted_test

ACME HS dissociation: an adaptable cell fixation-dissociation method for fresh-frozen human adrenal gland tissues for scRNA-seq
by Anastasia Shcherbakova | Marina Utkina | Ruslan Deviatiiarov | Alina Ryabova | Lilia Urusova | The National Medical Research Center for Endocrinology | The National Medical Research Center for Endocrinology | Graduate School of Medicine, Juntendo University, Tokyo, Japan | The National Medical Research Center for Endocrinology | The National Medical Research Center for Endocrinology
Abstract ID: 236
Event: BGRS-abstracts
Sections: [Sym 1] Section “Genome-wide transcriptomics (differential gene expression)”

Sample preparation is a crucial step in single-cell RNA sequencing (scRNA-seq) of human tissues to obtain biologically relevant data. Current methods encounter challenges in obtaining high-quality single-cell suspensions, especially in tissues where live cell isolation is difficult. To address this gap, we introduce ACME HS (ACetic (acid)-MEthanol Hight Salt), an optimized approach that facilitates the retrieval of intact and whole cells from fresh-frozen tissues. In this study, we conducted a comparative analysis of cellular heterogeneity in datasets of single cells from the human adrenal medulla obtained from ACME HS, enzymatic dissociation, and nuclei isolation.