Accepted_test

Features of extracellular matrix remodeling in human uveal melanoma
by Iuliia Taskaeva | Anastasia Shatruk | Nataliya Bgatova | Alena Yeremina | Aleksander Trunov | Valeriy Chernykh | Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Ge-netics, Siberian Branch of the Russian Academy of Sciences | Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Ge-netics, Siberian Branch of the Russian Academy of Sciences | Research Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Ge-netics, Siberian Branch of the Russian Academy of Sciences | S. N. Fyodorov Federal State Institution National Medical Research Center Intersectoral Research and Technology Complex “Eye Microsurgery” Ministry of Health of the Russian Federation | S. N. Fyodorov Federal State Institution National Medical Research Center Intersectoral Research and Technology Complex “Eye Microsurgery” Ministry of Health of the Russian Federation | S. N. Fyodorov Federal State Institution National Medical Research Center Intersectoral Research and Technology Complex “Eye Microsurgery” Ministry of Health of the Russian Federation
Abstract ID: 103
Event: BGRS-abstracts
Sections: [Sym 9] Section ”Interstitial space and long extravascular drainage/transport pathways”

The extracellular matrix (ECM) represents a network of macromolecules that maintain the integrity of tissues and organs. ECM remodeling during carcinogenesis is a complex process of degradation and renewal of ECM components under the influence of factors secreted by tumor cells and matrix metalloproteinases (MMPs) produced by cancer-associated fibroblasts to stimulate the mechanisms of angiogenesis, metastasis, evasion of immune surveillance and drug resistance development. Studying the structural features and processes of ECM reorganization may contribute to the development of new approaches to the uveal melanoma (UM) treatment. The aim of this study was to evaluate the expression of markers associated with ECM remodeling in UM. Immunohistochemical staining of choroidal and tumor samples from patients with UM (n=15) and analysis of the expression levels of proteins associated with ECM degradation (αSMA, Collagen type I, FN1, MMP2, MMP14, TIMP2) were performed. An increase in the area of staining of αSMA(+) zones in the intratumoral region was shown. A decrease in the expression levels of collagen type I was noted. The studied tumor samples were characterized by a high expression of proteins associated with the degradation of the extracellular matrix – fibronectin, MMP2, MMP14 and TIMP2. The results obtained indicate an active process of ECM remodeling in UM, which may affect the invasive ability and metastatic spread of tumor cells.