Accepted_test

Distinctive hypothalamic gene expression profiles in hypertensive ISIAH and normotensive WAG rats exposed to a single short-term restraint stress
by Redina O. | Oshchepkov D. | Makovka Yu. | Fedoseeva L. | Seryapina A. | Markel A. | Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences | Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences | Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences; Novosibirsk State University | Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences | Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences | Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences; Novosibirsk State University
Abstract ID: 109
Event: BGRS-abstracts
Sections: [Sym 5] Section “Population and evolutionary genetics/genomics of wild and domestic animals”

The study was aimed to identify the strain-specific neural networks providing differences in hypothalamic response to stress in hypertensive ISIAH and normotensive WAG rats. The experiment was carried out on three-month-old male hypertensive ISIAH/Icgn and normotensive WAG/GSto-Icgn rats. Transcriptome sequencing (RNA-Seq) of the hypothalamus was performed on 4 groups of rats: 1) ISIAH_control; 2) WAG_control; 3) ISIAH_stress; 4) WAG_stress. The restraint stress procedure consisted of placing the rat in a tight wire mesh cage for 2 hours. Distinctive differential expression of a large number of genes was observed in hypothalamic profiles of hypertensive ISIAH and normotensive WAG rats exposed to single short-term restraint stress. Functional annotation of DEGs made it possible to identify the biological processes that change most significantly in each rat strain. The strain-specific key DEGs and hypothalamic gene networks were identified.