Accepted_test

Distinctive hypothalamic gene expression profiles in hypertensive ISIAH and normotensive WAG rats exposed to a single short-term restraint stress
Authors:
Redina O., Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences
Oshchepkov D., Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences
Makovka Yu., Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences; Novosibirsk State University
Fedoseeva L., Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences
Seryapina A., Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences
Markel A., Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences; Novosibirsk State University
Abstract ID: 109
Event: BGRS-abstracts
Sections: [Sym 5] Section “Population and evolutionary genetics/genomics of wild and domestic animals”

The study was aimed to identify the strain-specific neural networks providing differences in hypothalamic response to stress in hypertensive ISIAH and normotensive WAG rats. The experiment was carried out on three-month-old male hypertensive ISIAH/Icgn and normotensive WAG/GSto-Icgn rats. Transcriptome sequencing (RNA-Seq) of the hypothalamus was performed on 4 groups of rats: 1) ISIAH_control; 2) WAG_control; 3) ISIAH_stress; 4) WAG_stress. The restraint stress procedure consisted of placing the rat in a tight wire mesh cage for 2 hours. Distinctive differential expression of a large number of genes was observed in hypothalamic profiles of hypertensive ISIAH and normotensive WAG rats exposed to single short-term restraint stress. Functional annotation of DEGs made it possible to identify the biological processes that change most significantly in each rat strain. The strain-specific key DEGs and hypothalamic gene networks were identified.