Accepted_test
The content of abscisic acid, zeatin, and indolylacetic acid was studied by enzyme immunoassay in the apical eyes of tubers of the original and Bt-gene transformed potato plants of the Superior variety. At the same time, the effect of the essential microelements zinc and boron on the content of phytohormones was studied. On the third day of tuber germination, transgene eyes contained 1.9 times more ABA, but significantly less IAA (2.5 times). In terms of zeatin content, the eyes of tubers of transgenic plants practically did not differ from the original ones. Soaking tubers for 6 hours in solutions of ZnSO4 and H3BO3 specifically changed the hormonal status of tuber eyes of transgenic and non-transformed plants. The trace element zinc significantly reduced the ABA content and increased the level of cytokinins compared to boron, regardless of transformation. The auxin content in transgenes increased to the same extent under the influence of microelements, while boron showed a greater effect in the original transgenes. Microelements increased the ratio of stimulating phytohormones to ABA. Zinc was the most effective in transgenes.