Accepted_test

Mesenchymal stem cell proliferation and NO production under cyanobacteria and microalgae condition
by Lykov Alexander | Gevorgiz Ruslan | Zheleznova Svetlana | Uvarov Ivan | Poveshchenko Olga | Research Institute of Clinical and Experimental Lymphology - filial of the Institute Cytology and Genetics | A.O. Kovalevsky Institute of Biology of the Southern Seas | A.O. Kovalevsky Institute of Biology of the Southern Seas | Koltsovskaya Veterinary Clinic, | Research Institute of Clinical and Experimental Lymphology - filial of the Institute Cytology and Genetics
Abstract ID: 121
Event: BGRS-abstracts
Sections: [Sym 9] Section “Molecular Pathology, Diagnostics, and Therapeutics”

It has been demonstrated that Chlorella vulgaris aqueous extract can function in lieu of fetal embryonic serum in the culture of Chinese hamster ovary cells and mesenchymal stem cells. Diatoms, cyanobacteria, and green microalgae  were all used to extract physiologically active compounds into DMSO. The impact of DMSO extracts of cyanobacteria and microalgae on mesenchymal stem cells (MSCs) from rat and human bone marrow was assessed. Proliferative potential (MTT method) and NO production (Griss reagent) by human or rat mesenchymal stem cells were assessed. Except for N. shiloi (human MSCs) and phycocyanin (rat MSCs), it was observed that adding extracts of microalgae, cyanobacteria, and fucoxanthin to the nutritional medium promotes the proliferation/viability of human and rat MSCs. Furthermore, it was discovered that, with the exception of N. shiloi and L. cf. ectocarpi, extracts of microalgae, cyanobacteria, and phycocyanin increase NO generation in human MSCs in a dose-dependent manner. When phycocyanin, microalgae, and cyanobacteria extracts were added to the nutritional medium, rat MSCs were likewise accountable for enhanced NO generation; however, no obvious dose-dependent effect was observed. Since the capacity of these microalgae and cyanobacteria species to be examined had not been before, our results of boosting the proliferation and secretory capabilities of human and rat MSCs with DMSO extracts of these microorganisms are unique.