Accepted_test

Differential expression of two alkane oxidation systems in response to hydrocarbon exposure in Tsukamurella tyrosinosolvens PS2
by Romanova Valeriya | Kupriyanova Elena | Grigoryeva Tatiana | Laikov Alexander | Kazan Federal University | Kazan Federal University | Kazan Federal University | Kazan Federal University
Abstract ID: 140
Event: BGRS-abstracts
Sections: [Sym 8] Section “Microbial communities of natural and anthropogenic habitats”

Most alkane-oxidizing bacteria contain a hydroxylase system, which is encoded by the alkB gene cluster. In addition, it has been shown that cytochrome P450 is involved in the oxidation of alkanes with a chain length of C5-C16. The studied strain Tsukamurella tyrosinosolvens PS2 contains in its genome two alternative alkane oxidation systems (AlkB and P450). RT-qPCR analysis showed that in strain PS2, 2 genes can be induced by medium- and long-chain alkanes. Notably, alkB gene expression was increased in the presence of long-chain alkanes. Meanwhile, medium-chain alkanes significantly increased the expression level of the P450 gene. Thus, a switch between two genes is observed depending on the length of the alkane chain. Understanding new mechanisms of regulation of complex alkane-oxidizing systems will increase the efficiency, productivity and rate of degradation of alkanes.