Accepted_test

The goal of this work was to identify the genomic rearrangements emerged from the adaptive laboratory evolution of the Rhodococcus rhodochrous bacterium and led to the acquirement of improved biocatalysts strains for acrylic monomers production. In the mutation spectrum determined by means of comparative genomic analysis, we observed two types of mutations associated with genes whose expression is significant for the biosynthesis of target products: IS mediated knockouts and large deletions. The findings allow to significantly narrow down the list of genes responsible for the target enzymatic activities of biocatalyst strains, and to suggest the major role of two regulatory genes in flexible switching of the aforementioned phenotype.