Accepted_test

РНК-связывающие свойства многофункционального белка NUCB1 и его возможная роль в регуляции трансляции /RNA-Binding capacity of multifunctional NUCB1 protein and its proposal role in translation regulation

by Olga Kostareva | IPR RAS

Abstract ID: 18

Event: BGRS-abstracts

Sections: [Sym 11] Section “Transcription, splicing, translation”

Nucleobindin 1 (Calnuc, NUCB1) is a highly conserved multi-domain DNA- and calcium-binding eukaryotic protein. NUCB1 is involved in the regulation of many processes. Recently we have shown that NUCB1 is capable to form high-affinity complexes with some of human micro-RNAs which are associated with system lupus erythematosus (SLE) and EMT. We assume that RNA-binding ability of NUCB1 has the functional significance and could be develop on gene expression regulation at the translational level. The purpose of current study was investigation of contribution of different NUCB1 domains to the RNA interaction.Five truncated forms of NUCB1 protein have been produced and RNA-binding ability for three of them has been estimated by surface plasmon resonance technique.Calcium-binding domain of NUCB1 protein is shown to sufficient to the interaction with RNA, moreover the interaction is calcium-independently. Only one article is devoted to involvement of EF-hand motif of VILIP protein in RNA-binding, but that interaction is calcium-dependent. Apparently, NUCB1 protein recognize RNA using a unique, not yet studied mechanism, and could be involved in the regulation of translation of mRNAs via interaction with microRNAs.