Accepted_test

Molecular genetic response to oxidative stress differs in hypothalamus of hypertensive ISIAH and normotensive WAG rats

by Yulia V. Makovka | Dmitry Yu. Oshchepkov | Larisa A. Fedoseeva | Arcady L. Markel | Olga E. Redina | Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia | Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia | Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia | Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia | Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia

Abstract ID: 180

Event: BGRS-abstracts

Sections: [Sym 5] Section “Population and evolutionary genetics/genomics of wild and domestic animals”

An important feature in the pathogenesis of hypertension is oxidative stress, which occurs under the influence of various exogenous and/or endogenous factors, including psycho-emotional stress. The molecular genetic mechanisms of the response to oxidative stress depending on the genotype and hypertensive state have not been fully studied. The purpose of this work was to establish the inter-strain hypothalamic differences in the expression of genes associated with the response to oxidative stress in hypertensive ISIAH and normotensive WAG rats after exposure to a single two-hour restraint (emotional) stress.

Functional annotation of DEGs revealed two groups of genes representing a response to oxidative stress common to the two strains and oxidative stress response genes specific to ISIAH rats. Among the common DEGs, the genes Nos1, Ppargc1a, Abcc1, Srxn1, Cryab, Hspb1 and Fosl1 changed the transcription level most significantly. The gene set enrichment analysis for promoter binding sites for transcription factors revealed two terms associated with binding sites for the transcription factor CREB1 and the glucocorticoid receptor (NR3C1) in the hypothalamus of both rat strains, suggesting that these transcription factors may play a key role in regulation of the response to oxidative stress regardless of the genotype/strain differences and hypertensive state of the rats. A specific response to oxidative stress in hypertensive ISIAH rats is associated with induction of Fos gene expression, which was confirmed by q-PCR.