Accepted_test

This study examines interactions between Aeromonas hydrophila and plasma proteins of cultured rainbow trout Oncorhynchus mykiss. The aim of the study was to investigate protein-protein interactions between bacteria and host using high-throughput mass spectrometry, the study. Growth dynamics, morphology and protein profiles of A. hydrophila were evaluated upon exposure to trout plasma from different populations. In order to identify molecules with antimicrobial activity, a comparative proteomic analysis of fish plasma samples with different antimicrobial activity was performed. In addition, trout proteins interacting with live A. hydrophila in vivo and adaptive metabolic rearrangements of the pathogen treated with fish plasma were investigated, to elucidate the mechanisms of action of possible fish antimicrobial factors. This research contributes to the understanding of the natural antibacterial activity of fish plasma and to the search for new antibacterial proteins in biological fluids.