Accepted_test

In the recent decade in-vitro fertilization (IVF) was widely adopted throughout the world, and more than 10 million children were born with IVF assistance. However, this reproductive technology manipulates with most vulnerable stages of organism development — gametes, zygotes and early blastocysts, which may cause variety of effects in latter life. Apart from more serious complications like obstetric complications and developmental disorders, IVF has more subtle effects, on cardiovascular systems and neurohumoral regulation. One such effect, consistently observed throughout studies is increased body mass in IVF offspring. In this work we examine how IVF conceived young adult (3 months) male mice differ from their naturally conceived counterparts in their activity and feeding behavior, using behavioral time series obtained from PhenoMaster behavioral monitoring system. We show that IVF-conceived mice are more active and sleep less both during day and night, and have higher respiratory exchange ratio during the day than naturally conceived counterparts. It is likely that these differences contribute to faster body mass gain that we observe the IVF group.