Accepted_test

Influence of breast cancer and surgical treatment of breast cancer on the amount of miR-21, miR-27a, miR-221 and miR-429 in the thymus
by Kazakov Oleg | Kabakov Aleksey | Poveshchenko Alexander | Cherkas Valeria | Bodrova Natalia | Kononchuk Vladislav | Gulyaeva Lyudmila | Scientific institution of clinical and experimental lymphology – branch of federal state budgetary scientific institution Federal Research Center Institute of Cytology and Genetics Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia | Scientific institution of clinical and experimental lymphology – branch of federal state budgetary scientific institution Federal Research Center Institute of Cytology and Genetics Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia | Scientific institution of clinical and experimental lymphology – branch of federal state budgetary scientific institution Federal Research Center Institute of Cytology and Genetics Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia | Scientific institution of clinical and experimental lymphology – branch of federal state budgetary scientific institution Federal Research Center Institute of Cytology and Genetics Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia | Federal State Budgetary Educational Institution of Higher Education Novosibirsk State Agrarian University, Novosibirsk, Russia | Research Institute of Molecular Biology and Biophysics (RIMBB) - structural subdivision of federal state budgetary scientific institution Federal Research Center for Fundamental and Translational Medicine, Novosibirsk, Russia | Research Institute of Molecular Biology and Biophysics (RIMBB) - structural subdivision of federal state budgetary scientific institution Federal Research Center for Fundamental and Translational Medicine, Novosibirsk, Russia
Abstract ID: 20
Event: BGRS-abstracts
Sections: [Sym 9] Section “Molecular Pathology, Diagnostics, and Therapeutics”

The purpose of the study was to compare the expression levels of miR-21, miR-27a, miR-221, miR-429 in the thymus of breast cancer and after surgical treatment of breast cancer. This work was carried out on mature female Wistar rats. Breast cancer was modeled by 5-fold subcutaneous injection of N-methyl-N-nitrosourea with an interval of 7 days into the area of the 2nd breast on the right. Surgical treatment of breast cancer was carried out 6 months from the start of breast cancer induction. To determine the levels of pro-oncogenic miR-21, miR-27a, miR-221 and tumor-suppressive miR-429 in biological samples, real-time RT-PCR was performed on a CFX96 amplifier, small RNA U6 was used as a reference gene. In the breast cancer group without treatment, compared with the intact group, the amount of miR-21 in the thymus was reduced in 7.3-fold, miR-27a in 10-fold, miR-221 in 4.3-fold. After surgical treatment of breast cancer, compared with breast cancer without treatment, the amount of miR-21 in the thymus increased in 3-fold, miR-27a in 3.9-fold. The expression of tumor-suppressive miR-429 both in the group with breast cancer without treatment and after surgical treatment of breast cancer remains at the level of the intact group. In breast cancer, a decrease in the amount of miR-21, miR27a, miR-221 in the thymus may be due to an immune response to tumor growth and accidental involution of the thymus. After surgical treatment of breast cancer, compared with breast cancer without treatment, the amount of miR-21 and miR-27a in the thymus increases.